Prostein (P501S; SLC45A3; PCANAP6)Catalogue number: P501S-0.5
The rabbit monoclonal antibody ZR9 is well suited to detect Prostein (SLC45A3) in prostate tissue. It is very specific and usually overexpressed in poorly differentiated prostate carcinoma. Human prostein is a 553 aa protein identified by cDNA library subtraction and subsequent high throughput microarray a screening of prostate cancer. Prostein has multiple transmembrane domains. Prostein has been shown to be uniquely expressed in normal and cancerous prostatic tissues. In immunohistochemistry, prostein is expressed by the vast majority of normal and malignant prostatic tissues, regardless of grade and metastatic status. No protein expression is detected in other normal and malignant tissue samples representing the great majority of essential tissues and tumors. In particular, prostein is expressed in most of poorly differentiated prostatic carcinoma, including small cell prostate carcinoma. Prostein is more specific and sensitive for prostatic carcinomas than PSA and PSAP. Human Prostein, SLC45A3 (Solute Carrier Protein Family 45 Member 3), PCANAP6 (Prostate Cancer Associated Protein 6).
Immunogen: Synthetic N-terminal Peptide of human Prostein
Ion exchange chromatography purified antibody in PBS, BSA, sodium azide (0.09%)*. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.
Purification Method: Ion exchange chromatography purified antibody in PBS, BSA, sodium azide (0.09%)*. Use antibody dilution buffer (e.g. Art. No. PU002) containing sufficient protein and preservative.
Secondary Reagents: Any secondary system containing anti-rabbit IgG may be used.
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1:50-1:100
Pre-Treatment: In formaldehyde fixed tissue unmasking of antigen by heat pre-treatment is required. We recommend the application of our unmasking fluid G on glycol basis (Art. No. DE007) or EDTA solution pH 9 (Art.-No. DE006) approx. 30 min in pressure cooker or micro wave oven. Citrate buffer is also suitable, but gives less intensity in staining and changes the cell morphology more than the above mentioned unmasking methods.
Positive Control: Prostate, Prostate Carcinoma
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Xu J., Kalos M., Stolk J.A., Zasloff E.J., Zhang X., Houghton R.L., Filho A.M., Nolasco M., Badaro R., Reed S.G. (2001) Identification and characterization of prostein, a novel prostate-specific protein. Cancer Res. 61:1563-1568(2001) [PubMed] [Europe
Database Name: UniProt
Accession number: Q96JT2 - S45A3_HUMAN
Species Accession: Human
Prostein (P501S; SLC45A3; PCANAP6)