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CA 19-9, Sialyl-Lewis a


Catalogue number: CA199-1

Clone121 SLE
IsotypeIgM
Units1 ml
HostMouse
Application ELISA
IHC (cryostat and paraffin sections)
IRMA
Background
CA 19-9 antibody reacts strongly positive with most tumour cell lines of gastrointestinal carcinoma including adenocarcinoma of the stomach, intestine, and pancreas. Positive reactivity was also found in transitional cell carcinoma of the bladder, adenocarcinoma of the endometrium and the gallbladder as well as in papillary carcinoma of the thyroid glands. Last but not least positive reactivity of lung carcinoma must be mentioned (adenocarcinoma, bronchio-alveolar carcinoma, squamous epithelial carcinoma, and SCLC). Sialyl Lewis a antigen is an oncofetal antigen, which appears during early development of the gastrointestinal tract. After birth it is still detectable in ductus and secretion of intestinal glands, respiratory and reproductive tract. In normal tissue Sialyl Lewis a (CA 19-9) is found in ductal epithelia of the breast, kidney, salivary glands, lung, colon, bile duct, liver, pancreas and prostate gland. Sialyl Lewis a antigen (Cancer Antigen CA 19-9).

Positive Control
Colon carcinoma

Pre-Treatment
In paraffin section no pre-treatment is required, however heat induced antigen retrieval may improve the staining. Each laboratory should select it's own optimum staining and pre-treatment conditions.

Immunogen
Immunodiffusion precipitate of ovarian cystic mucins with NS19-9-antibody

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Working Concentration
(liquid conc.) 1:10 - 1:50

Concentration
50 µg/ml

Incubation Time
60 min at RT

Secondary Reagents
As secondary reagents we recommend the use of biotinylated anti-mouse IgM antibody (Art. No. BA-2020) in combination with streptavidin conjugates or ABC systems.

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.

Applications
ELISA, IRMA, IHC (cryostat and paraffin sections)

Cross Reactivity
Human

Storage
2-8°C

References
1. Gatalica Z., and Miettienen M. (1994) Distribution of carcinoma antigens CA 19-9 and CA 15-3: an immunohistochemical study of 400 tumors. Appl. Immunohistochem. 2(3); 205ff. 2. Tomazic A., and Pegan V. (2000) Preoperative staging of periampullar cancer with US, CT, EUS and CA 19-9. Hepatogastroenterol. 47(34); 1135-1137. 3. Tabata T., Takeshima N., Tanaka N., Hirai Y., and Hasumi K. (2000) Clinical value of tumor markers for early detection of recurrence in patients with cervical adenocarcinoma and adenosquamous carcinoma. Tumour Biol. 21(6); 375-380. 4. Dalgleish A.G. (2000) Tumour markers in malignancies. CA19.9 is useful in several cancers. B.M.J. 321(7257); 380

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CA 19-9, Sialyl-Lewis a

$585.00

SKU: CA199-1 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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