Bombesin, Gastrin Releasing PeptideCatalogue number: GRP001
The antibody against Bombesin is well suited to detect the neuropeptide in different tissues of vertebrates especially in amphibia (brain, peripheral nerves, neuroendocrine cells of the lung). Due to its sequence homology with GRP (Gastrin Releasing Peptide) it is also suitable for the detection of GRP in mammals including humans. Beside its normal occurrence in the intestinal tract, GRP is found in carcinoids of the lung and in other neuroendocrine tumours. The tetradecapeptide is a biological active neuropeptide in the CNS and in peripheral nerves of amphibian. It has effects on thermoregulation and the motility of the intestine. In neurons of the CNS it may co-exist with substance P, in the intestine however different cells were located immunohistochemically for substance P and Bombesin / GRP (Costa et al. 1984). Bombesin (pGlu-Gln-Arg-Leu-Gly Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH2) and related peptides e.g. Gastrin Releasing Peptide (mammalian homolog aa sequence bold).
Immunogen: Synthetic Bombesin (frog) conjugated with bovine thyroglobulin
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Amphibians, Mammalians
Incubation Time: IHC(P) 60 min at RT or 2-8°C over night
Working Concentration: (liquid conc.) 1:50
Pre-Treatment: No protease pre-treatment required. Improvement may be achieved by HIER.
Positive Control: Amphibian brain, gut (peripheral nerves) fetal lung
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. Costa M., Furness J.B., Yanaihara N., Yanaihara C., and Moody T.W. (1984) Cell and Tissue res. 235; 285-293 2. Gomi T., Kikuchi Y., Adriensen D., Timmermans J.-P., De Groodt-Lassel M.H.A. et al. (1994) Immunocytochemical survey of the neuroepithelial endocrine system in the respiratory tract of the Tokyo salamander, Hynobis nebulosus tokyoensis TAGO. Histochemistry 102; 425-431.
Bombesin, Gastrin Releasing Peptide