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Mouse anti Human Actin (N-Terminal)


Catalogue number: X2672M

CloneBA3R
IsotypeIgG2b
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Chicken
Human
Mouse
Rabbit
Rat
Application Dot Blot
ELISA
WB
Western Blotting
Background
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, -actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Positive Control
Recognizes native and denatured forms of β-Actin (~42kDa)

Immunogen
-Actin N-terminal peptide-KLH conjugate

Purification Method
Protein A/G Chromatography

Concentration
See vial for concentration

Formulation
10mM PBS, pH 7.2, with 0.05% Sodium Azide

Functional Analysis
Western Blotting

Applications
WB (with ECL): 1:1000-10,000 dilutions (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L., Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA. Nucleic Acids Res. 12:1687-1696(1984) 2. Nakajima-Iijima S., Hamada H., Reddy P., Kakunaga T.,Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns. Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985) 3. Procaccio V., Salazar G., Ono S., Styers M.L., Gearing M., Davila A., Jimenez R., Juncos J., Gutekunst C.-A., Meroni G., Fontanella B., Sontag E., Sontag J.-M., Faundez V., Wainer B.H. A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78:947-960(2006) [PubMed: 16685646] [Abstract]

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Human Actin (N-Terminal)

$180.00