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Mouse anti Human Actin (N-Terminal)

Catalogue number: X2672M

CloneBA3R
IsotypeIgG2b
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Chicken
Human
Mouse
Rabbit
Rat
Application Dot Blot
ELISA
Western Blotting

Background
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, -actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Source

Immunogen: -Actin N-terminal peptide-KLH conjugate

Product

Formulation: 10mM PBS, pH 7.2, with 0.05% Sodium Azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
WB (with ECL): 1:1000-10,000 dilutions (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Recognizes native and denatured forms of β-Actin (~42kDa)

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Ship Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

References
1. Ponte P., Ng S.Y., Engel J., Gunning P., Kedes L., Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA. Nucleic Acids Res. 12:1687-1696(1984) 2. Nakajima-Iijima S., Hamada H., Reddy P., Kakunaga T.,Molecular structure of the human cytoplasmic beta-actin gene: interspecies homology of sequences in the introns. Proc. Natl. Acad. Sci. U.S.A. 82:6133-6137(1985) 3. Procaccio V., Salazar G., Ono S., Styers M.L., Gearing M., Davila A., Jimenez R., Juncos J., Gutekunst C.-A., Meroni G., Fontanella B., Sontag E., Sontag J.-M., Faundez V., Wainer B.H. A mutation of beta -actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. Am. J. Hum. Genet. 78:947-960(2006) [PubMed: 16685646] [Abstract]

Protein Reference(s)

Database Name: SwissProt

Accession number: P60709

Species Accession: Human


Safety Datasheet(s) for this product:
Sodium Azide
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Mouse anti Human Actin (N-Terminal)

$184.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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