Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to human lysozyme

  • Product Code: RAHu-Lys-Bio
  • Size: 1 ml
  • Price (USD): $393

Product Code

RAHu-Lys-Bio		 Quantity:      

Product Name

Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to human lysozyme

Size

1 ml

Price (USD)

$393

Background

The reactivity of the antiserum is restricted to lysozyme. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal human milk and tears a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified lysozyme. No reaction is obtained with any other plasma protein component or serum. In immunocytochemical and immunohistochemical techniques for the detection of lysozyme at the cellular and subcellular level in appropriately treated cell and tissue substrates. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions for histochemical and cytochemical use are usually between 1:10 and 1:50.

Host

Rabbit

Isotype

IgG

Species Reactivity

Human

Product Usage

Biotin-conjugated IgG fraction of polyclonal Rabbit antiSerum to Human lysozyme.

Product Type

Primary Antibodies

Applications

Immunocytochemistry, Immunohistochemistry (Frozen), ELISA, Western Blotting

Fields of Interest

Cardiovascular

Product Source

Highly purified recombinant human lysozyme. Lysozyme, a bacteriolytic enzyme, has a molecular weight of about 15,000. Its concentration in plasma is low; in milk the concentration is reported to be between 5 and 220 mg/ml. Also tears contain a considerable amount of lysozyme. Freund's complete adjuvant is used in the first step of the immunization procedure.

Product Formulation

Biotin-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.

Product Specificity

The antiserum does not cross-react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

Storage Conditions

Lyophilized at +4