The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal dog serum; to prepare an immunoadsorbent for the production of Ig-free dog serum, plasma or non-Ig preparations.
Precipitating polyclonal Rabbit antiSerum to Dog IgG, heavy and light chains.
Fields of Interest
Purified normal IgG isolated from pooled dog serum. Freund
Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion with the following results: Bovine - Cat + Chicken - Duck - ferret + Goat - Guinea Pig + Hamster + Horse + Human + Mouse + Monkey + pigeon - Rat + Sheep - Swine + Turkey -
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4