P53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Alterations of the p53 tumor suppressor gene are considered critical events in multistage carcinogenesis of a wide range of human cancers. The mutations found in sporadic cancer are commonly missense mutations that inactivate the tumor suppressor activity of p53. These mutant proteins are much more stable than the normal p53 protein, frequently adopt an altered conformation and accumulate within the nucleus of the malignant cells. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma. Positive nuclear staining with p53 antibodies has been reported to be a negative prognostic factor in amongst others breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma.
ELISA,Flow cytometry,Immunoblotting,Immunocytochemistry,Immunofluorescence,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
Fields of Interest
DO-1 is a mouse monoclonal IgG2a antibody derived by fusion of mouse myeloma Sp2/0-Ag14 cells with spleen cells from BALB/c mice immunized with recombinant human wild type p53 protein expressed in E. coli.
Store at 4°C or for longer periods at -20°C in small aliquots.