The reactivity of the antiserum is directed to the Fc and Fab subunits of the major immunoglobulins. It does not react with any non-Ig protein in chicken serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins; as secondary antibody to precipitate the immunoglobulin in normal chicken serum.
Precipitating polyclonal Goat antiSerum to Chicken immunoglobulins, heavy and light chains
Fields of Interest
Purified normal Ig fractions isolated from pooled chicken serum. FreundÕs complete adjuvant is used in the first step of the immunization procedure.
Delipidated, heat inactivated, lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4¡C; prolonged storage at or below -20¡C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4¡C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4¡ C--at least 10 years reconstituted at or below -20¡ C--3-5 years reconstituted at +4¡ C--7 days