Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE)

  • Product Code: GIC-201
  • Size: 50 Tests
  • Availability: In Stock In Stock
  • Price (USD): $209

Product Code

GIC-201		 Quantity:      

Data Sheet

Product Name

COMBI IC Reagent: IgG Negative Control (FITC) and IgG Negative Control (PE)

Size

50 Tests

Price (USD)

$209

Background

This ready to use Negative Control reagent contains a combination of fluorescein and phycoerythrin conjugated mouse immunoglobulin molecules of IgG1 isotype, which have been selected on the basis of their binding characteristics: no specific binding to human intracellular or cell surface antigens, same low range of nonspecific binding to human leukocytes as other COMBI-IC-Reagents. Like all other COMBI-IC-reagents, this reagent should be used in combination with our FIX&PERM_ Cell Permeabilization Kit (Cat.No. GAS-002) These isotype control IgG1 are suitable as negative controls to be used in combination with COMBI-IC reagents for the: - Enumeration of Myeloid Cells - Analysis of Myeloid Differentiation Stage - Enumeration of B-cells and Precursors - Enumeration of T-cells and Precursors - Analysis of Leukemia Cells - Analysis of Immunodeficiency States Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation.

Clone Name

VI-AP and VI-AP

Host

Mouse

Isotype

IgG1

Product Type

Negative Control

Applications

Permeabilization and Staining Procedure - In combination with our Permeabilization Kit FIX&PERM_ (Cat. No. GAS-002) intracellular isotype controls can be easily stained in cell suspensions. - For each sample to be analyzed add 50

Product Formulation

PBS pH 7.2, 1% BSA, 0.05% NaN3

Storage Conditions

Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

References

J. L. Clarke, W. Watkins, J Biol Chem 271, 10317 (1996).
R. N. Knibbs et al., J Cell Biol 133, 911 (1996).
B. Kniep et al., J Biochem (Tokyo) 119, 456 (1996).
A. J. Wagers, L. M. Stoolman, R. Kannagi, R. Craig, G. S. Kansas, J Immunol 159, 1917 (1997).
M. Noguchi, N. Sato, H. Sugimori, K. Mori, K. Oshimi, Leuk Res 25, 847 (2001).
W. M. Watkins, J. L. Clarke, Adv Exp Med Biol 491, 231 (2001).