Each staining performed with specific monoclonal antibodies should be paralleled by a staining with an appropriate istotype matched control antibody, in order to be able to control for non-specific binding.
The COMBI-REAGENT-Negative Control permits to estimate the degree of non-specific binding of isotype matched immunologbulins to leukocytes via e.g. Fc-receptors. It enables the expert to set flow cytometric parameters accordingly.
Results must be put within the context of other diagnostic tests as well as the clinical history of the patient by a certified professional before final interpretation. Analyses performed with this antibody should be paralleled by positive and negative controls. If unexpected results are obtained, which cannot be attributed to differences in laboratory procedures, please contact us.
4H1-A7 and VI-AP
Direct Immunofluorescence (Staining Procedure)
Nordic-MUbio fluorochrome labeled antibodies are designed for use with either whole blood or isolated mononuclear cell (MNC) preparations.
Proposed staining procedure for whole blood in short:
- For each sample add 50
PBS pH 7.2, 1% BSA, 0.05% NaN3
Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8