Exalpha Biologicals, Inc.

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FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
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Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
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COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE)

  • Product Code: GCT-201
  • Size: 50 Tests
  • Availability: In Stock In Stock
  • Price (USD): $265

Product Code

GCT-201		 Quantity:      

Data Sheet

Product Name

COMBI IC Reagent: Mouse anti CD45 (FITC) and Mouse anti CD14 (PE)

Size

50 Tests

Price (USD)

$265

Background

The CD45 molecule is typically expressed at high levels on all hematopoietic cells. CD45 is a major component of the glycocalix of these cells and can be expressed in different isoforms. Antibody VIT200 recognizes a pan CD45 epitope, which is expressed on all hematopoietic cells. CD14 is a GPI-anchored molecule expressed by virtually all human monocytes and macrophages and

Clone Name

VIT200 and MEM18

Host

Mouse

Isotype

IgG2a and IgG1

Species Reactivity

Human

Product Type

Primary Antibodies

Applications

Staining Procedure Direct Immunofluorescence (Staining Procedure) Nordic-MUbio fluorochrome labeled antibodies are designed for use with either whole blood or isolated mononuclear cell (MNC) preparations. Proposed staining procedure for whole blood in short: - For each sample add 50

Product Formulation

PBS pH 7.2, 1% BSA, 0.05% NaN3

Storage Conditions

Nordic-MUbio monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8

References

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