Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with FITC

  • Product Code: GAM-IgG(Fc)-FITC
  • Size: 1 ml
  • Price (USD): $453

Product Code

GAM-IgG(Fc)-FITC		 Quantity:      

Product Name

Goat anti Mouse IgG1 IgG2a IgG2b IgG3 (Fc specific), conjugated with FITC

Size

1 ml

Price (USD)

$453

Background

The reactivity of the antiserum is directed to the Fc subunit of the IgG molecule. The antiserum contains antibodies to subclass-specific determinants as well as to determinants shared by two or more subclasses of IgG. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against normal mouse plasma, serum, and immunoglobulin fractions, no reaction is obtained with IgA, IgM and IgG/Fab fragments or any other serum protein. In immunocytochemical and immunohistochemical staining of IgG at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgG antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of mouse origin known to be of the IgG isotype in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:40 and 1:120, depending on the method used.

Host

Goat

Species Reactivity

Mouse

Product Usage

Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Mouse IgG, Fc specific

Product Type

Secondary Antibodies

Applications

ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence

Fields of Interest

Veterinary

Product Source

Purified normal IgG, including all known subclasses, isolated from pooled mouse serum. Freund

Product Formulation

FITC-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Product Specificity

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been tested in double radial immunodiffusion anti several sera with the following results: cow - Duck - Horse - Rat + Cat - Guinea Pig+ Monkey - Sheep - Chicken - Human - pigeon - Swine - Dog - Hamster

Storage Conditions

The lyophilized conjugate is shipped at ambient temperature and may be stored at +4