Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Goat anti Bovine IgG (heavy and light chains)

  • Product Code: GAB-IgG(H+L)-7S
  • Size: 10 mg
  • Price (USD): $184

Product Code

GAB-IgG(H+L)-7S		 Quantity:      

Product Name

Goat anti Bovine IgG (heavy and light chains)

Size

10 mg

Price (USD)

$184

Background

The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in bovine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the userÕs own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:50 and 1:250; in ELISA and comparable non-precipitating antibody-binding assays between 1:500 and 1:5,000.

Host

Goat

Species Reactivity

Bovine

Product Usage

Purified IgG fraction of polyclonal Goat antiSerum to Bovine IgG, heavy and light chains

Product Type

Secondary Antibodies

Applications

Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting

Fields of Interest

Veterinary

Product Source

Purified normal IgG isolated from pooled bovine serum. FreundÕs complete adjuvant is used in the first step of the immunization procedure.

Product Formulation

Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Product Specificity

Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Storage Conditions

The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4¡C; prolonged storage at or below -20¡C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20¡C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4¡C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4¡ C--at least 10 years. Reconstituted at or below -20¡ C--3-5 years. Reconstituted at +4¡ C--7 days