The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in bovine serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user
Purified IgG fraction of polyclonal Goat antiSerum to Bovine IgG, heavy and light chains
Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting
Fields of Interest
Purified normal IgG isolated from pooled bovine serum. Freund
Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
The lyophilized IgG fraction is shipped at ambient temperature and may be stored at +4