Chicken anti-native Green Fluorescent Protein
- Product Code: Bii-nGFPabC1-100
- Size: 0.1 ml
- Availability:
In Stock - Price (USD): $304
Product Code
Data Sheet
Product Name
Chicken anti-native Green Fluorescent Protein
Size
0.1 ml
Price (USD)
$304
Background
Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.
Clone Name
Polyclonal
Host
Chicken
Species Reactivity
n/a
Product Usage
Aequoria Victoria (native) GFP, recombinant (E.Coli) and native GFP-fusion proteins, and all variants of GFP tested.
Product Type
Primary Antibodies
Applications
The antibody is suitable for the detection of GFP by western blotting and ELISA.
Optimal antibody dilutions for the different applications should be determined by titration. Suggested dilutions for immunoblotting: 1:1000-1:5000, using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. For ELISA: 1:2,000
Product Formulation
Each vial contains 100
Storage Conditions
Store at 4oC, or in small aliquots at -20oC.
References
1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.2. UniProt: P42212 (A.victoria)
