Exalpha Biologicals, Inc.

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Exalpha Biologicals, Inc. is now a US distributor for Nordic-Mubio products

Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

Nordic-MUbio is one of the leading antibody companies developing antibodies for zebrafish research – we currently have over 50 antibodies validated for immunofluorescence in zebrafish.

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Latest References

Akazawa, Y., et al. "Endoplasmic Reticulum Stress Contributes to Helicobacter Pylori VacA-Induced Apoptosis" PLoS ONE 8(12): e82322

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Homa, N.J., et al. "Epstein-Barr Virus Induces Global Changes in Cellular mRNA Isoform Usage That Are Important for the Maintenance of Latency" J. Virol., 2013, 87, 12291-12301

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Chicken anti-native Green Fluorescent Protein

  • Product Code: Bii-nGFPabC1-100
  • Size: 0.1 ml
  • Availability: In Stock In Stock
  • Price (USD): $304

Product Code

Bii-nGFPabC1-100		 Quantity:      

Data Sheet

Product Name

Chicken anti-native Green Fluorescent Protein

Size

0.1 ml

Price (USD)

$304

Background

Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.

Clone Name

Polyclonal

Host

Chicken

Species Reactivity

n/a

Product Usage

Aequoria Victoria (native) GFP, recombinant (E.Coli) and native GFP-fusion proteins, and all variants of GFP tested.

Product Type

Primary Antibodies

Applications

The antibody is suitable for the detection of GFP by western blotting and ELISA. Optimal antibody dilutions for the different applications should be determined by titration. Suggested dilutions for immunoblotting: 1:1000-1:5000, using colorimetric detection (Peroxidase/TMB), with higher dilution possible using more sensitive detection methods. For ELISA: 1:2,000

Product Formulation

Each vial contains 100

Storage Conditions

Store at 4oC, or in small aliquots at -20oC.

References

1. Chalfie M, Tu Y, Euskirchen G, Ward WW, Prasher DC. (1994) Green fluorescent protein as a marker for gene expression. Science 263: 802-805.

2. UniProt: P42212 (A.victoria)