Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Chicken anti-native Green Fluorescent Protein

  • Product Code: Bii-nGFPabC1-100
  • Size: .1 ml
  • Price (USD): $304

Product Code

Bii-nGFPabC1-100		 Quantity:      

Product Name

Chicken anti-native Green Fluorescent Protein

Size

.1 ml

Price (USD)

$304

Background

Green fluorescent protein (GFP) is a 27kD protein which was originally identified in the photo organs of Aequorea victoria (A. victoria) jellyfish. GFP is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. In A.victoria, calcium ions bind and activate the protein aequorin causing the release of blue fluorescence, which is then absorbed by GFP resulting in the release of green fluorescence. In the laboratory, the GFP protein has been used extensively as a reporter molecule to label, and study, cellular and subcellular proteins in living cells using a wide range of biological applications, including oncology, cardiovascular diseases, brain research and embryology, just to name a few. For the quantitation of the expression of a specific protein, tagged with GFP in these model systems, antibodies to GFP have proven to be of value in immunoblotting studies and ELISA protocols.

Host

Chicken

Species Reactivity

n/a

Product Usage

The GFP antiserum is directed against native green fluorescent protein from A.victoria jellyfish. The antibody recognises not only the native GFP, but also recombinant protein (E.Coli), native GFP-fusion proteins and all variants of GFP tested. Cross reactivity with E.coli. proteins is minimal.

Product Type

Primary Antibodies

Applications

Western Blotting, ELISA

Product Source

The anti-GFP antibody is a chicken polyclonal antiserum obtained from whole egg yolk of chickens immunised with highly purified native GFP derived from Aequorea victoria jellyfish.

Product Formulation

Each vial contains 100µl 1 mg/ml polyclonal antiserum in 10 mM TRIS buffer pH 8.0, containing 0,15 mM NaCl and 0.02% sodium azide. The antibodies were purified by ammonium sulfate precipitation.

Product Specificity

Aequoria Victoria (native) GFP, recombinant (E.Coli) and native GFP-fusion proteins, and all variants of GFP tested.

Storage Conditions

Store at 4oC, or in small aliquots at -20oC.