Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

CD Antibodies

Single Color Reagents

Conjugates available in 100 tests/vial size. Pure antibody available in 100 µg size.

Please click on catalog number below to open product page.

Product Description
Pure
FITC
Biotin
RPE
APC
Cy5RPE*
Isotype
CD2**
T & NK Subset (cln T6.3)
-
IgG2a k
CD3
T Cells (cln M2AB)
IgG1 k
CD4**
Helper/Inducer T cell (cln 7E14)
IgG1 k
CD5
T and B cells (cln M28623)
-
-
IgG2a k
CD7
T cells (cln MG34)
-
-
IgG2a k
CD8**
Suppressor/Cytotoxic T cells (cln 17D8)
IgG1 k
CD10
CALLA (cln RXB.14)
-
-
-
-
IgG1 k
CD11a**
LFA-1 alpha (cln OFYS)
-
-
IgG2a k
CD11b
Mac-1 (cln F6.2)
-
-
IgG2a k
CD13
Pan Myeloid cell, 150 kDa (cln E735)
-
-
-
IgG1 k
CD14
Monocyte/Macrophage, Mr 53 kDa (cln FWKW)
-
-
IgG2b k
CD15
Myelomonocytic cells (cln ARE)
-
-
-
-
IgM
CD15-NA
Non-Aggregating CD15 (cln MCS-1)
-
-
-
-
-
IgG3
CD16
Natural Killer Cell, Mr 50-65 kDa (cln J5511)
-
-
IgG1 k
CD18**
LFA-1 beta
-
-
IgG1 k
CD19
B cells (cln 1G9)
IgG1 k
CD20
Pan B cells (cln DFA-7)
-
-
-
IgG1 k
CD22
Mature B cell-Hairy cell Leukemia (cln 5.8HK)
-
-
-
IgG2b k
CD23
preB Lymphocytes (cln LVNC-4)
-
-
-
IgG1 k
CD25**
IL-2R/p55 (cln ITYV)
-
IgG1 k
CD26
Dipeptidyl Peptidase (DPP) (cln EUG-6)
-
-
-
IgG1 k
CD27
Human Nerve Growth Factor (NGF) cells (cln DEN-3)
-
-
-
IgG1 k
CD28
Human T cell subset, Tp44 (cln B-23)
-
-
IgG1 k
CD33
Human Myelomonocytic cell Mab
-
-
-
-
IgG1 k
CD34***
Human Stem Cell Mab
-
-
-
IgG1 k
CD35
Human CR-1 Mab
-
-
IgG1 k
CD38
Proliferation Cells (cln TME-2)
-
-
-
IgG1 k
CD41a
Platelet Megakaryocytes (cln 96.2C1)
-
-
-
-
-
IgG1 k
CD42a
Platelet GPIX (cln ESS)
-
-
IgG1 k
CD44
Pgp-1 HCAM (cln 7F1)
-
-
-
IgG1 k
CD45
Mr 180 to 220 kDa (cln BHPT-1)
IgG1 k
CD45RA
Leukocyte Common Antigen (cln BHKG-2)
-
-
-
IgG1 k
CD54
ICAM (cln D3.6)
-
-
IgG2b k
CD56
Natural Killer Cell Mab (cln C5.9)
-
-
IgG2b k
CD61
Platelet gpIIIa cell (cln 43.PL)
-
-
-
IgG1 k
CD62L
LECAM-1, L-selectin, DREG-56 (cln 1H3)
-
-
-
IgG2a k
CD69
Activated T, B, NK and Macrophages (cln SMM)
-
-
-
IgG1 k
CD71
Transferin Receptor (cln BGX.24)
-
-
-
IgG1 k
CD95
fas/Apo 1 (cln 3.22)
-
-
-
IgG1 k
CD103
alpha E integrin, subset CD8+ (cln AX.14)
-
-
-
IgG1 k
CD117
c-kit Ligand (cln NU-c-kit)
-
-
-
IgG1 k
HLA-DR** 
HLA-DR Class II B and T subset (cln 423L)
-
-
IgG2a k
Human IgG
Human IgG (cln ICO-106)
-
-
-
-
-
IgG1
V-PreB
CD179a, PreB Cell Receptor (cln B-MADD688)
-
-
-
-
-
IgM

 

* Portions of this product are manufactured under license from Carnegie Mellon University, US Patent No. 5268486

** Reacts with Macaca species monkey

*** Not available in the United States