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VEGFR-2 (KDR), human, biotinylated


Catalogue number: VEGR201-B

Clone4H3
IsotypeIgG1
Units50 µg
HostMouse
Application ELISA
FACS
Flow Cytometry
Background
The antibody against VEGFR-2 is well suited to detect the receptor in tissue fluids, culture media, and on the surface of different human cell types. The induction of angiognesis by VEGF-C and VEGF-A is mediated by VEGF-Receptors 2 and -3, while VEGF-B is bound by VEGF-Receptor-1 only. VEGF-D also acts trough VEGFR-3 in lymphangiogenesis and tumour metastasis. Human VEGFR-2/KDR.

Positive Control
bone marrow

Immunogen
Recombinant human KDR protein 110 kDa extracellular domain of VEGFR-2

Purification Method
Protein G purified antibody, lyophilized from TRIS/HCl buffer 0.1 M, 0.2 M NaCl, 0.02% NaN3, pH 7.4. Reconstitute with 0.1 ml sterile water  to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.

Working Concentration
(lyophilisate) ELISA 1-15 µg/ml; Western Blot 1-2 µg/ml; FACS 2-5 µg/ml

Concentration
50 µg

Secondary Reagents
As secondary reagents Avidin or Strepavidin conjugates are recommended. The label may be alkaline phosphatase (Ref. ZU051), peroxidase (Ref. ZU054).

Product
Protein G purified antibody, lyophilized from TRIS/HCl buffer 0.1 M, 0.2 M NaCl, 0.02% NaN3, pH 7.4. Reconstitute with 0.1 ml sterile water  to get an antibody concentration of 1 mg/ml. Dilution buffers are depending on the type of application.

Applications
ELISA, FACS

Cross Reactivity
Human, other species not tested

Storage
-20°C

References
1. Rustemeyer P, Wittkowski W, Greve B, Stehling M. Flow-cytometric identification, enumeration, purification, and expansion of CD133+ and VEGF-R2+ endothelial progenitor cells from peripheral blood. J Immunoassay Immunochem. 2007;28(1):13-23. 2. Zhao B, Smith G, Cai J, Ma A, Boulton ME. VEGF-C Promotes Survival of Retinal Vascular Endothelial Cells via VEGFR-2. Br J Ophthalmol. 2006 Aug 30; 3. Neumuller J, Neumuller-Guber SE, Lipovac M, Mosgoeller W, Vetterlein M, Pavelka M, Huber J. Immunological and ultrastructural characterization of endothelial cell cultures differentiated from human cord blood derived endothelial progenitor cells. Histochem Cell Biol. 2006 Dec;126(6):649-64. 4. Benzinger et al. (2000) BBA 1466; 71

Caution
These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals.

Safety Datasheet(s) for this product:

VEGFR-2 (KDR), human, biotinylated

$549.00

SKU: VEGR201-B Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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