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Uroplakin III

Catalogue number: UP3001-G

Units5 ml
Application IHC (P)
IHC in cryostat sections may require formaldehyde fixation
Western Blotting
Together with the Uroplakins UP Ia, UP Ib and UP II, UP III is a constituent of the Asymmetric Unit Membrane of urothelial Umbrella cells. It is a 47 kDa glycoprotein, which is thought to be a specific marker for differentiated urothelia. UP III is found in surface membranes of urothelial cells of the kidney, bladder and urinary collection tract. In paraffin sections app. 60% of transitional cell carcinoma stain focally with Uroplakin III antibodies. Yet no Uroplakin has been found in non-urothelian carcinoma. Therefore it seems to be a specific marker of urothelial cells in metastases of unknown origin of the primary tumours. Uroplakin III membrane protein.

Positive Control

Pre-treatment of paraffin sections with proteinase K or trypsin

Asymmetric Unit Membrane (AUM) preparation from bovine bladder

Purification Method
Protein A purified antibody lyophilized from PBS containing 0.09% NaN3 and 0.5% BSA after reconstitution with 1 ml distilled water

Working Concentration
(RTU) neat

Incubation Time
60 min at RT or 18 hr at 2-8°C

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Protein A purified antibody lyophilized from PBS containing 0.09% NaN3 and 0.5% BSA after reconstitution with 1 ml distilled water

IHC (P), IHC in cryostat sections may require formaldehyde fixation, WB

Cross Reactivity
Human, cattle, rat, pig


1. Moll R, Wu XR, Lin JH, Sun TT (1995) Uroplakins, specific membrane proteins of urothelial umbrella cells, as histological markers of metastatic trasitional cell carcinomas. Am. J. Pathol. 147; 1383-1397. 2. Romith R, Jezernik K, Maera A (1998) Uroplakins and cytokeratins in the regenerating rat urothelium after sodium saccharin treatment. Histochem Cell Biol 109:263-269. 3. Kaufmann O, Volmering J, and Dietel M. (2000) Uroplakin III is a highly specific and moderately sensitive immunohistochemical marker for primary and metastatic urothelial carcinoma. Am. J. Clin. Pathol. 113; 683-687. 4. Ramos-Vara JA, Miller MA, Boucher M, Roudabush A, and Johnson GC. (2003) Immunohistochemical detection of uroplakin III, cytokeratin 7, and cytokeratin 20 in canine urothelial tumors. Vet. Pathol. 40(1); 55-62.

*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

Uroplakin III


SKU: UP3001-G Categories: ,


Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370