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Sheep anti Rabbit PhosphoglyceRate mutase


Catalogue number: NE131/PAb

ClonePolyclonal
Units1 mg
HostSheep
Background
The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition. This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques); to prepare an insoluble immuno-affinity adsorbent; for labelling with a marker of the customer’s own choice.

Formulation
Purified hyperimmune sheep IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran. No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Source
Phosphoglyceric mutase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Product
Purified hyperimmune sheep IgG antibodies lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2), stabilized with dextran. No preservative added, as it may interfere with the antibody activity. No foreign protein added. It is reconstituted by adding 0.5 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

Applications
Immunoprecipitation.

Cross Reactivity
Cross-reactivities against enzymes of other sources may occur but have not been determined.

Specificity
Affinity purified antibodies of polyclonal Sheep antiSerum to phosphoglyceric mutase from Rabbit muscle

Storage
The lyophilised PAb fraction is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

Safety Datasheet(s) for this product:

Sheep anti Rabbit PhosphoglyceRate mutase

$462.00

SKU: NE131/PAb Categories: ,