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Sheep anti Human ELK rec tyr Kinase (Eph B1 Ext.)


Catalogue number: S100P

Synonyms
Eph B1 (ELK receptor) EXT; Tyrosine-protein kinase receptor EPH-2; Ephrin type B receptor 1; HEK6

IsotypeIgG
Product Type Polyclonal Antibody
Units250 µg
HostSheep
Species reactivity Human
Application Immunoprecipitation
Product Form
Unconjugated

Background
The immunogen used for the anti-human ephB1 (Elk receptor) -EXT region was a GST fusion protein ephB1 (Elk receptor) corresponding to amino acid sequence 16-351 of the N terminal, extracellular region, and having a molecular weight of 116 kD. ephB1, previously known as Elk(eph-like kinase), is a receptor tyrosine kinase of the highly tissue restricted family of eph proteins. EphB1 and other ephB family members are type 1 membrane spanning proteins, comprised of immunoglobulin, fibronectin type III, and cysteine rich subdomains in the ecto domain, and the single uninterrupted cytoplasmic tyrosine kinase domain upstream of a carboxyterminal sterile alpha motif (SAM) domain. ephB family proteins bind ephrins of the B class, ligands that are also transmembrane spanning proteins capable of transmitting signals. ephB1 is expressed predominantly in developing neural structures in embryos, and in vascular epithelium of kidney, and other tissues. Upon binding to alternatively oligomerized ephrin B1, ephB1 signals regulation of cell attachment and cellcell assembly. Members of this protein family are implicated in neuronal and vascular cell targeting.

Positive Control
Human brain

Immunogen
A GST fusion protein ephB1 (Elk receptor) corresponding to amino acid sequence 16-351 of the N terminal, extracellular region, and having a molecular weight of 116 kD

Purification Method
Ammonium Sulfate Precipitation

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Applications
Application: 1-10 ug/ml for Western Blotting and 10-20 ug/ml for Immunoprecipitation.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Room Temperature

Product Stability
Antibodies are stable for one year from purchase if stored frozen

References
1. Stein, E., et al. 'Nck recruitment to Eph receptor, EphB1/ELK, couples ligand activation to c-Jun kinase.' J. Biol. Chem., 273, 1303-1308 (1998) 2. Han, D.C., et al. 'EphB1 associates with Grb7 and regulates cell migration.' J. Biol. Chem., 277, 45655-45661 (2002) 3. Vindis, C., et al. 'EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis.' J. Cell Biol., 162, 661-671 (2003)

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
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Sheep anti Human ELK rec tyr Kinase (Eph B1 Ext.)

$254.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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