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Mouse anti Gram Negative Bacteria DNA Adenine Methylase

Catalogue number: X1610M

CloneRB1
IsotypeIgG1
Product Type Monoclonal Antibody
Units100 µg
HostMouse
Species reactivity Gram Negative Bacteria
Application Western Blot

Background
DNA adenine methylase (DAM) is an enzyme that methylates adenine residues in the 4-base pair DNA sequence, GATC. This methylation reaction has been shown to be crucial in a variety of cellular processes in bacteria. These include (i) the control of initiation of DNA replication (GATC occurs 11 times in the highly conserved bacterial origin of replication sequence); (ii) methyl-directed mismatch repair; (iii) control of transposition of certain transposable DNA elements; and (iv) control of gene expression. With regard to gene expression, the recent report by Heithoff et al. (Science; 284:967-970; 1999) showed that the deletion of the DAM gene rendered Salmonella typhimurium totally avirulent. Antibody can be used for Western blotting (1-5 µg/ml). Other applications not yet evaluated. Optimal concentration should be evaluated by serial dilutions. The antibody reacts with the recombinant protein as produced by E.coli. We have no data to support to its reactivity with wild type DAM protein.

Synonyms: DAM; DNA adenine methyltransferase; Deoxyadenosyl-methyltransferase

Source

Immunogen: Recombinant DNA Adenine Methylase protein.

Product

Product Form: Unconjugated

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide

Purification Method: Protein A/G Chromatography

Concentration: See vial for concentration

Applications
Antibody can be used for Western blotting (1-5 µg/ml). Other applications not yet evaluated. Optimal concentration should be evaluated by serial dilutions.

Functional Analysis: Western Blotting

Positive Control: Not available

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Product Stability: Products are stable for one year from purchase when stored properly

Ship Conditions: Ship at ambient temperature, freeze upon arrival

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

References
1. Pogolotti, AL Jr., et al. 'On the mechanism of DNA-adenine methylase.' J Biol Chem, 1988, 263, 7461-7464. 2. Heithoff, D.M., et al. ‘An essential role for DNA adenine methylation in bacterial virulence.’ Science, 284, 967-970

Protein Reference(s)

Database Name: SwissProt

Accession number: P0AEE8

Species Accession: E.coli


Safety Datasheet(s) for this product:
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Mouse anti Gram Negative Bacteria DNA Adenine Methylase

$254.00


Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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