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Chicken anti Clostridium difficile Toxin B

Catalogue number: ACdTB

Product Type Primary Antibodies
Units0.1 mg
HostChicken
Application ELISA
Western Blotting

Source
Chickens were immunized with highly purified Toxin B purified from Clostridium difficile. After multiple immunizations in Freund's adjuvant, eggs were collected and the IgY purified. Anti-Clostridium difficile Toxin B was immunoaffinity purified using the immunogen immobilized on a solid support.

Product

Product Form: Affinity purified

Formulation: Phosphate buffered saline‚ pH 7.2 with 0.075% sodium azide

Purification Method: Egg-yolk derived IgY Affinity Purified

Concentration: See vial for concentration.

Applications
This antibody is suitable for ELISA and Western Blotting procedures. By Direct ELISA, the half-point titre was 1:20,000 using a 2 ug/ml coating of Toxin B. This antibody has also been successfully used in cytotoxicity and neutralization assays (please see embedded image). Optimal working dilutions should be determined for your particular assay conditions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. 

References
1. Lei X-H and BR Bochner, 2013. Using Phenotype MicroArrays to Determine Culture Conditions That Induce or Repress Toxin Production by Clostridium difficile and Other Microorganisms. PLoS ONE 8(2): e56545. doi:10.1371/journal.pone.0056545 2. Bochner, B and X. Lei. 2015. Compositions and Methods to Inactivate and/or Reduce Production of Microbial Toxins. United States Patent Application 2015 0374786. 3. Tussey, L., Song, L. and S. Umlauf. 2016. Clostridium difficile Compositions and Methods of Use. United States Patent Application 2016016667.


Safety Datasheet(s) for this product:
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf

Chicken anti Clostridium difficile Toxin B

$264.00

SKU: ACdTB Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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