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CDX2, 1C7


Catalogue number: CDX002

Clone1C7
IsotypeIgG mix kappa
Units1 ml
HostMouse
Application IHC(C
P)
Background
The antibody against CDX2 is well suited for the detection of the product in cell nuclei and in tissue extracts. CDX2 is a homeobox-gen encoding for an intestinal transcription factor, which is exprimed by nuclei of intestinal epithelial cells especially in the rectum and duodenum. It is over-expressed in colorectal carcinoma and related metastases. It was also identified in metaplasia of the stomach and in carcinoma of the gut. CDX2 is not produced by normal mucous membranes of the stomach. CDX2, human.

Positive Control
Colon carcinoma

Pre-Treatment
Formalin-fixed tissue requires antigen unmasking in citrate buffer pH 6 (Art. No. DE000) 15-30 min at 100°C. Blocking of endogenous oxido-reductases with H2O2 1% 30 min., blocking of non-specific protein binding with SeaBlock (Art. No. PU083).

Immunogen
Recombinant human CDX2 AS 1-314 with GST-Tag. MYVSYLLDKDVSMYPSSVRHSGGLNLAPQNFVSPPQYPDYGGYHVAAAAAAAANLDSAQSPGPSWPAAYGAPLREDWNGYAPGGAAAAANAVAHGLNGGSPAAAMGYSSPADYHPHHHPHHHPHHPAAAPSCASGLLQTLNPGPPGPAATAAAEQLSPGGQRRNLCEWMRKPAQQSLGSQVKTRTKDKYRVVYTDHQRLELEKEFHYSRYITIRRKAELAATLGLSERQVKIWFQNRRAKERKINKKKLQQQQQQ

Purification Method
Purified IgG in PBS, pH 7.2, 0,09% sodium azide

Working Concentration
(liquid conc.) 3 µg/ml

Concentration
50 µg/ml

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Purified IgG in PBS, pH 7.2, 0,09% sodium azide

Applications
IHC(C, P)

Cross Reactivity
Human, others not tested

Storage
2-8°C

References
1. Barbareschi M., Murer B., Colby T.V., Chilosi M., Macri E., Loda M., Coglioni C. (2003) CDX-2 homeobox gene expression is a reliable marker of colorectal adenocarcinoma metastases to the lungs. Am J. Surg. Pathol. 27; 141-149 2. Werling R.W. Yaziji H., Bacchi C.E., Gown A.M. (2003) CDX2, a highly sensitive and specific marker of adenocarcinomas of intestinal origin: An immunohistochemical survey of 476 primary and metastatic carcinomas. Am. J. Surg. Pathol. 27; 303-310.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CDX2, 1C7

$569.00

SKU: CDX002 Categories: ,