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CD5, human


Catalogue number: CD5-2-1

CloneEP77
IsotypeIgG
Units1 ml
HostRabbit
Application FACS
Flow Cytometry
IHC(C
P)
Background
The antibody to CD5 is used for the classification of lymphoid neoplasm. CD5 is exprimed by the majority of T-cell lymphoma and is also found on B-cells in some cases of chronic lymphatic leukaemia and centrocytic lymphoma. Normally it is not found in other forms of lymphoid neoplasm e.g. follicular lymphoma, Hairy Cell Lymphoma and Large Cell Lymphoma. CD5 is a lymphocyte surface molecule with a molecular weight of 67 kDa. It is exprimed by 95% of thymocytes and app. 72% of peripheral blood lymphocytes. In lymph nodes the antigen is detected in the T-cell region mainly. CD5 is over-expressed in leukaemia and T-cell lymphoma and may also be detected in a subpopulation of activated B-cells. Human CD5 molecule.

Positive Control
Tonsil

Pre-Treatment
Paraffin sections are pre-treated with 1 mM EDTA (pH 8.0) in an autoclave (2 min under full pressure, cooling 15-20 min.)

Immunogen
Synthetic Protein, corresponding to a sequence of human CD5 molecule

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art.No. PU002.

Working Concentration
(liquid conc.) 1:10-1:25

Incubation Time
60 min at RT

Secondary Reagents
We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art.No. PU002.

Applications
IHC(C, P), FACS

Cross Reactivity
Human

Storage
2-8°C

References
1. Jones N.H., Clabby M.L., Dialynas D.P. et al. (1986) Isolation of complementary DNA clones encoding the human lymphocyte glycoprotein T1/Leu-1. Nature 323: 346-349. 2. Kasaian M.T., Ikematsu H. and Casali P. (1991) CD5+ B-Lymphocytes (43250). Proceedings of the Society for Experimental Biology and Medicine 197; 226-241. 3. Chan J.K.C., Banks P.M., Cleary M.L. (1994) A proposal for classification of lymphoid neoplasms (by the international lymphoma study group). Histopathology 25; 517-536.

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD5, human

$664.00

SKU: CD5-2-1 Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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