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CD274, PD-L1


Catalogue number: CD274-0.5

CloneZR3
IsotypeIgG
Units0.5 ml
HostRabbit
Application IHC(C
P)
Background
Programmed cell death ligand 1 (PD-L1) also known as cluster of differentiation (CD274) or B7 homolog 1 (B7-H1) is a type 1 transmembrane protein involved in the regulation of cellular and humoral immune responses. The interaction of PD-L1 with its receptor PD-1 provides both stimulatory and inhibitory signals in regulating T cell activation and tolerance during pregnancy, tissue allograft, autoimmune disease and malignant transformation. The binding of PD-L1 to PD-1 induces apoptosis or exhaustion in activated T cells, and blockade of this interaction has been shown to enhance the antitumor activity of T cells. PD-L1 is frequently over expressed in placenta, and many human tumours such as melanoma, diffuse large B-cell lymphoma, and carcinomas of the lung, bladder, breast, kidney and colon. Human CD274, PD-L1.

Positive Control
Placenta, lung carcinoma (NSCLC)

Pre-Treatment
Unmasking with Unmasking fluid E (Art. No. DE006) pH 9.0 or Unmasking fluid T (DE005) pH 8.0

Immunogen
KLH-conjugated linear peptide from human PD-L1

Purification Method
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.**

Working Concentration
(liquid conc.) 1:50-1:100

Product
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide.**

Applications
IHC(C, P)

Cross Reactivity
Human

References
1. Ishida, Agata, Shibahara et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. The EMBO journal, Vol. 11, Issue 11, pp. 3887-95 (PubMed). 2. Wang W, et al. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi (2016) IFN-? up-regulates PD-L1 expression in human placenta mesenchymal stem cells and enhances cell ability to induce the differentiation of IL-10+ T cells from cord blood- and peripheral blood-derived T cells. PMID 26927379 3. Benjamin J. Chen, Bjoern Chapuy, Jing Ouyang, et al. (2013) PD-L1 Expression Is Characteristic of a Subset of Aggressive B-cell Lymphomas and Virus-Associated Malignancies Clinical Cancer Research. CCR-13-0855

Caution
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!

Safety Datasheet(s) for this product:

CD274, PD-L1

$632.00

SKU: CD274-0.5 Categories: ,