CD10, humanCatalogue number: CD10-1
The antibody agains CD10 is mainly used for the paenotypic analysis of leukaemia. It is used as a marker for the ALL cell subset, which is considered as a cell type with relative good prognosis. It is used for differentiation of acute lymphoblastic leukaemia (ALL) from acute myeloic leukaemia (AML). CD10 is an integral membrane protein with a 25 aa cytoplasmatic domain. The extracellular domain contains 12 cysteine residues and 6 positions for N-glycosylation. CD10, also called CALLA (Common acute lymphoblastic leukaemia antigen) is exprimed by a number of cell types, including lymphoid progenitor cells, B-cell line ALL and neutrophils. B-cells in the germinal center, some other types of leukaemia and non-lymphoid tissues like tubular renal epithelia also exprime this glycoprotein. CD10, human lymphocyte cell surface antigen 100 kDa.
Immunogen: Recombinant human CD10
Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Purification Method: Antibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**. The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. BIOLOGO Art .No. PU002.
Secondary Reagents: We recommend the use of BIOLOGO's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005).
Species Reactivity: Human
Incubation Time: 60 min at RT
Working Concentration: (liquid conc.) 1:10-1.50 depending on fixation and secondary reagents
Pre-Treatment: Formaldehyde-fixed material should be treated with Unmasking Solution C, citrate buffered solution pH 6 (Art. No. DE000) at 96-100°C
Positive Control: Tonsil
*These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
1. H. Zola (1995) B2 CD10 Workshop Panel Report. In: Schlossman S.F. et al.eds. Leucocyte Typing V. White cell differentiation antigens. Oxford University Press, Oxford, N.Y., Tokyo pp. 505-507.