Mouse anti Human Caspase-6
Catalogue number: X1173M
Synonyms
Cysteine-requiring Aspartate Protease-6; Apoptotic protease Mch-2
Clone | MCH2 14 1-190 |
Isotype | IgG |
Product Type | Monoclonal Antibody |
Units | 100 µg |
Host | Mouse |
Species reactivity | Human |
Application | Western Blot |
Unconjugated
Background
Caspase-6 (Cysteine-Requiring Aspartate Proteases) is part of a family of intracellular cysteine proteases that cleave their substrates after aspartic acid residues. These proteases play an integral role in inducing apoptosis in cells. Procaspase-6 (Mch2), a member of the ICE/ced-3 subfamily, is an inactive proenzyme that is activated to form caspase-6 by proteolytic cleavage at certain aspartic acid residues. During cleavage, the N-terminal is removed and the proenzyme is converted into a large (p18) and small (p11) subunits. Caspase-6 has two isoforms, and , produced by alternative splicing. Over-expression of the isoform of caspase-6 without its prodomain can induce apoptosis. The isoform does not seem to display proteolytic activity. Together with caspases-3 and -7, the isoform of caspase-6 is classified as an effector/execution caspase. Caspase-3, caspase-8 and caspase-10 can cleave procaspase-6. Active caspase-6 cleaves several other proteins such as lamins, NuMa and Keratin 18. A possible cleavage of caspases-8 and -10 in cytochrome-C dependent apoptosis was reported recently.
Positive Control
MCF-7 cell lysate
Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-6 protein and mouse myeloma cells.
Purification Method
Protein A/G Chromatography
Concentration
See vial for concentration
Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Functional Analysis
Western Blotting
Applications
Detects proenzyme form of human Caspase-6 by Western blot. Optimal concentration should be evaluated by serial dilutions.
Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Ship Conditions
Ship at ambient temperature, freeze upon arrival
Product Stability
Products are stable for one year from purchase when stored properly
References
1. Kidd, V.J., Proteolytic activities that mediate apoptosis. Annu. Rev. Physiol. 1998, 60, 533-573 2. Fernandes – Alnemri, T., et al., Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family. Cancer Res. 1995, 55, 2737-2742 3. Orth, K., et al., The CED-3/ICE-like protease Mch2 is activated during apoptosis and cleaves the death substrate lamin A. J. Biol. Chem. 1996, 271, 16443 4. Hirata, H., et al., Caspases are activated in a branched protease cascade and control distinct downstream processes in Fas-induced apoptosis. J. Exp. Med. 1998, 187, 587-600 5. Slee, E. A., et al., Ordering the cytochrome c-initiated caspase cascade: hierarchial activation of caspases-2, -3, -6, -7, -8 and -10 in a caspase-9-dependent manner. J. Cell Biol., 144, 281 (1999) 6. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16
Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Safety Datasheet(s) for this product:
Sodium Azide
/wp-content/uploads/2018/07/Antibody-SDS-with-Sodium-AzideV2.pdf
Mouse anti Human Caspase-6
$254.00