Mouse anti Human Caspase-6

Catalogue number: X1173M

Synonyms
Cysteine-requiring Aspartate Protease-6; Apoptotic protease Mch-2

Clone	MCH2 14 1-190
Isotype	IgG
Product Type	Monoclonal Antibody
Units	100 µg
Host	Mouse
Species reactivity	Human
Application	Western Blot

Product Form
Unconjugated

Background
Caspase-6 (Cysteine-Requiring Aspartate Proteases) is part of a family of intracellular cysteine proteases that cleave their substrates after aspartic acid residues. These proteases play an integral role in inducing apoptosis in cells. Procaspase-6 (Mch2), a member of the ICE/ced-3 subfamily, is an inactive proenzyme that is activated to form caspase-6 by proteolytic cleavage at certain aspartic acid residues. During cleavage, the N-terminal is removed and the proenzyme is converted into a large (p18) and small (p11) subunits. Caspase-6 has two isoforms, and , produced by alternative splicing. Over-expression of the isoform of caspase-6 without its prodomain can induce apoptosis. The isoform does not seem to display proteolytic activity. Together with caspases-3 and -7, the isoform of caspase-6 is classified as an effector/execution caspase. Caspase-3, caspase-8 and caspase-10 can cleave procaspase-6. Active caspase-6 cleaves several other proteins such as lamins, NuMa and Keratin 18. A possible cleavage of caspases-8 and -10 in cytochrome-C dependent apoptosis was reported recently.

Positive Control
MCF-7 cell lysate

Immunogen
Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-6 protein and mouse myeloma cells.

Purification Method
Protein A/G Chromatography

Concentration
See vial for concentration

Formulation
Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis
Western Blotting

Applications
Detects proenzyme form of human Caspase-6 by Western blot. Optimal concentration should be evaluated by serial dilutions.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Ship at ambient temperature, freeze upon arrival

Product Stability
Products are stable for one year from purchase when stored properly

References
1. Kidd, V.J., Proteolytic activities that mediate apoptosis. Annu. Rev. Physiol. 1998, 60, 533-573 2. Fernandes – Alnemri, T., et al., Mch2, a new member of the apoptotic Ced-3/Ice cysteine protease gene family. Cancer Res. 1995, 55, 2737-2742 3. Orth, K., et al., The CED-3/ICE-like protease Mch2 is activated during apoptosis and cleaves the death substrate lamin A. J. Biol. Chem. 1996, 271, 16443 4. Hirata, H., et al., Caspases are activated in a branched protease cascade and control distinct downstream processes in Fas-induced apoptosis. J. Exp. Med. 1998, 187, 587-600 5. Slee, E. A., et al., Ordering the cytochrome c-initiated caspase cascade: hierarchial activation of caspases-2, -3, -6, -7, -8 and -10 in a caspase-9-dependent manner. J. Cell Biol., 144, 281 (1999) 6. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

Sodium Azide
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