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BrdU Fluorescent Immunohistochemistry Kit – FL488


Catalogue number: X2839K

Synonyms
BrdU (Bromodeoxyuridine) Immunofluorescence Kit

Product Type Immunofluorescence Kit
Units50 Slides
Application Immunohistochemistry (Paraffin & Frozen)
Background
Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labor intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.
A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators (1-8). In these methods bromodeoxyuridine (BrdU), a thymidine analog replaces [3H] thymidine. BrdU is incorporated, into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells, which are actively synthesizing DNA.
Exalpha Biologicals BrdU Immunofluorescence Kit involves incorporation of BrdU into proliferating cells, in vivo or in vitro, and fluorescent staining of these cells which is achieved using a biotinylated anti-BrdU antibody followed by Streptavidin-FL Conjugate

Concentration
See vial for concentration

Functional Analysis
Immunohistochemistry

Applications
Optimal concentration should be evaluated by serial dilutions.

Storage
Store kit at -20ºC until first use. Store at 4-8ºC thereafter.

Ship Conditions
Ship on gel ice, freeze upon arrival

Product Stability
Products are stable for 6 months from purchase when stored properly

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Kit Manual
Click here to download the Kit Manual

Safety Datasheet(s) for this product:

BrdU Fluorescent Immunohistochemistry Kit – FL488

$376.00

SKU: X2839K Categories: ,

Brand

Contact us

Exalpha Biologicals, Inc.
2 Shaker Road, Unit B101
Shirley, MA 01464
Phone: 978-425-1370
Email: info@exalpha.com

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