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A431 cells stimulated with EGF


Catalogue number: X1003

Synonyms
A431 stimulated with EGF control cell lysate

Product Type Control Lysate
Units100 µl
Application Western Blot
Product Form
Unconjugated

Background
A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

Concentration
See vial for concentration

Formulation
Provided as solution in Laemmli electrophoresis sample buffer. Samples need to be boiled for 3-5 minutes, before loading the gel.

Functional Analysis
Western Blotting

Applications
Suggested use: A volume of 10-25ul is suggested for immunobloting.

Storage
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Ship Conditions
Room Temperature

Product Stability
Reagents are stable for the period shown on the vial label when stored properly

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Safety Datasheet(s) for this product:

A431 cells stimulated with EGF

$59.00

SKU: X1003 Categories: ,