Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

References

X1165P Farnesyl
1. Clase, A.C., et al, 'The Pseudorabies Virus Us2 Protein, a Virion Tegument Component, Is Prenylated in Infected Cells' Journal of Virology 2003, 77, , 12285-12298


X1370M Heregulin/ZNDF/GGF/Neuregulin
1. Fujimoto, H., et al. ?Increased Cellular Distribution of Vimentin and Ret in the Cingulum of Rat Offspring After Developmental Exposure to Decabromodiphenyl Ether or 1,2,5,6,9,10-Hexabromocyclododecane.? J. Toxicol. Pathol., 26, 119-129 (2013)


0DR2 HLA-DR FITC
1. Weidmann, A., et al, 'Proteolytic Cleavage of the Fusion Protein but Not Membrane Fusion Is Required for Measles Virus-Induced Immunosuppression In Vitro' Journal of Virology 2000, 74, , 1985-1993


0G14 IgG1 PE
1. Luo, T., et al., 'Human Immunodeficiency Virus Type 1 Nef-Induced CD4 Cell Surface Downregulation Is Inhibited by Ikarugamycin' Journal of Virology 2001, 75, , 2488-2492


F105P KSHV/HHV8 (cyclin)
1. Jarviluoma, Annika et al, 'KSHV viral cyclin binds to p27KIP1 in primary effusion lymphomas' Blood 2004, 104, 10, 3349-3354


F100P KSHV/HHV8 (mal 62/ORF 62)
1. Watanabe, T., et al, 'Kaposi's Sarcoma-Associated Herpesvirus Latency-Associated Nuclear Antigen Prolongs the Life Span of Primary Human Umbilical Vein Endothelial Cells' Journal of Virology 2003, 77, , 6188-6196


T105P LEF-1
1. Labbe, Etienne, et al., 'Association of Smads with lymphoid enhancer binding factor 1/T cell specific factor mediates coopertive signaling by the transforming growth factor-Beta and Wnt pathways' Proceedings of the National Acadamy of Science 2000, 97, 15, 8358-8363


X1076M LEF-1 all isoforms
1. Aoki, M., et al, 'Oncogenic transformation by -catenin: deletion analysis and characterization of selected target genes' Oncogene 2002, 21, , 6983=8991


T100M LEF-1beta catenin
1. Driskell. Ryan R., et al, 'Wnt-responsive element controls Lef-1 promotor expressions durig submucosal galnd morphogenesis' American Journal of Physiology Lung Cell Moleculatr Physiology 2004, 287, , 752-763
2. Li. Feng-Qian, et al., 'Lymphoid Enhancer Factor-1 Links Two Hereditary Leukemia Syndromes through Core-binding Factor-alpha Regulation of ELA2' Journal of Biological Chemistry 2004, 279, 4, 2873-2884
3. Kahler, Rachel A., et al., 'Lymphoid Enhancer Factor-1 and Beta Catenin Inhibit Runx2-dependent Transcriptional Activation of the Osteocalcin Promotor' Journal of Biological Chemicstry 2003, 278, 14, 11937-11944
4. Grueneberg, D.A., et al., 'A Functional Screen in Human Cells Identifies UBF2 as an RNA Polymerase II Transcription Factor That Enhances the


T105M LEF/TCF/HMG
1. Holnthoner, Wolfgang, et al., 'Fibroblast Growth Factor-2 Induces Lef/Tcf-dependent Trnscription in Human Endothelial Cells' Journal of Biological Chemistry 2002, 277, 48, 45847-45853
2. Willinger, T., et al, 'Human Naive CD8 T Cells Down-Regulate Expression of the WNT Pathway Transcription Factors Lymphoid Enhancer Binding Factor 1 and Transcription Factor 7 (T Cell Factor-1) following Antigen Encounter In Vitro and In Vivo' Journal of Immunology 2006, 176, , 1439-


X1089P LFC
1. Chiang, H., et al. ?GEF-H1 controls microtubule-dependent sensing of nuclei acids for antiviral host defenses.? Nature Immunology, Jan 2014, 15(1): 63-71


X1647P Lipid Phosphate Phosphatase Related Protein 2
1. Zhao, Yutong, et al, 'Lipid phosphate phosphatase-1 regulates lysophosphatidic acid-induced calcium release, NF- B activation and interleukin-8 secretion in human bronchial epithelial cells' Biochem Journal 2005, 385, , 493-502


X1648P Lipid Phosphate Phosphatase Related Protein 3
1. Zhao, Yutong, et al, 'Lipid phosphate phosphatase-1 regulates lysophosphatidic acid-induced calcium release, NF- B activation and interleukin-8 secretion in human bronchial epithelial cells' Biochem Journal 2005, 385, , 493-502


X1570P Lysophosphatidic Acid 2 (LPA2) Receptor (EDG4) CT
1. Yun, C. Chris, et al, 'LPA2 receptor mediates mitogenic signals in human colon cancer cells' American Journal of Cell Physiolgy 2005, 289, , C2-C11
2. Shida, D., et a, 'Aberrant expression of lysophosphatidic acid (LPA) receptors in human colorectal cancer' Laboratory Investigation 2004, 84, , 1352-1362


c174p Lysophosphatidic Acid Receptor 1 CT (EDG-2)
1. Hu, Xiaojie, et al, 'Lysophosphatidic acid (LPA) protects primary chronic lymphocytic leukemia (CLL) cells from apoptosis through LPA receptor activation of the anti-apoptotic protein AKT/PKB' Journal of Biological Chemistry 2005, 280, , 9498-9508
2. Shida, Dai, et al, 'Aberrant expression of lysophosphatidic acid (LPA) receptors in human colorectal cancer' Laboratory Investigation 2004, 84, , 1352-1362
3. Shida, Dai et al, 'Lysophosphatidic Acid (LPA) Enhances the Metastatic Potential of Human Colon Carcinoma DLD1 Cells through LPA1' Cancer Research 2003, 63, , 1706-1711


Z115M M13 bacteriophage (g3p)
1. Sakai, Keiko et al, 'Isolation and Characterization of Phage-Displayed Single Chain Antibodies Recognizing Nonreducing Terminal Mannose Residues. 1. A New Strategy for Generation of Anti-Carbohydrate Antibodies' Biochemistry 2007, 46, 1, 253-262
2. Qi,Cai et al, 'Phage M13KO7 detection with biosensor based on imaging ellipsometry and AFM microscopic confirmation' Virus Research 2009, 140, , 79-84
3. Tragoolpua, Khajornsak et al, 'Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells' BMC Biotechnology 2008, 8, 5, Online only


X1405M Microphthalmia Transcription Factor (MiTF)
1. Tshori, S., et al, 'Transcription factor MITF regulates cardiac growth and hypertrophy' Journal of Clinical Investigation 2006, 116, , 2673-2681
2. Yamada, K., et al. ?EWS/ATF1 expression induces sarcomas from neural crest?derived cells in mice.? J. Clin. Invest., 123, 600-610 (2013)
3. Hagglund, A.-C., et al. ?Canonical Wnt/ -Catenin Signalling Is Essential for Optic Cup Formation.? PLoS One, 8, e81158 (2013)
4. Nakano, T., et al. ?Self-formation of optic cups and storable stratified neural retina from human ESCs.? Cell Stem Cell (2012) 10, 771-785


X2397M Microphthalmia Transcription Factor (MiTF)
2. Yamada, K., et al. ?EWS/ATF1 expression induces sarcomas from neural crest?derived cells in mice.? J. Clin. Invest., 123, 600-610 (2013)


X2398M Microphthalmia Transcription Factor (MiTF)
2. Yamada, K., et al. ?EWS/ATF1 expression induces sarcomas from neural crest?derived cells in mice.? J. Clin. Invest., 123, 600-610 (2013)


X2409P Microphthalmia Transcription Factor (MiTF)
2. Yamada, K., et al. ?EWS/ATF1 expression induces sarcomas from neural crest?derived cells in mice.? J. Clin. Invest., 123, 600-610 (2013)


X2729P Microphthalmia Transcription Factor (MiTF)
2. Yamada, K., et al. ?EWS/ATF1 expression induces sarcomas from neural crest?derived cells in mice.? J. Clin. Invest., 123, 600-610 (2013)


X1001 myc (a.a.?s 410 - 419)
1. Ghavampour, S., et al. ?Transcriptional Profiling of Human Monocytes Identifies the Inhibitory Receptor CD300a as Regulator of Transendothelial Migration.? PLoS One, 2013, 8, e73981


L148M Nerve Growth Factor (NGF) (Neutralizing)
1. Cheng, Hsinlin T, et.al, 'Nerve Growth Factor Mediates Mechanical Allodynia in a Mouse Model of Type 2 Diabetes' Neuropathology Expwerimental Neurology 2009, 68, 11, 1229-1243
2. Cheng, H.T., et al. ?Nerve growth factor/p38 signaling increases intraepidermal nerve fiber densities in painful neuropathy of type 2 diabetes.? Neurobiol. Dis. (2012), 45, 280-287


L146M NGF (Nerve Growth Factor)
1. Wild, Kenneth D. et.al, 'Antibodies to Nerve Growth Factor Reverse Established Tactile Allodynia in Rodent Models of Neuropathic Pain without Tolerance' The Journal of Pharmacology and Experimental Therapeutics 2007, 322, , 282-287
2. Eldredge, Laurie C. etal, 'Abnormal sympathetic nervous system development and physiological dysautonomia in Egr3-deficient mice' Developemnt and Disease 2008, 135, , 2949-2957


X1719M Nitrotyrosine
1. Kim, G.S., et al. ? Induction of thioredoxin-interacting protein is mediated by oxidative stress, calcium and glucose after brain injury in mice.? Neurobiology of Disease, May 2012, 46(2): 440-449


7033 NuCycl PI Kit
1. Eriksson, M., et al, 'Recurrent de novo point mutations in lamin A cause Hutchinson-Gilford progeria syndrome' Nature 2003, 423, , 293-298


X1600P OGR1/GPR68
1. Iwai, K., et al. ?RGS18 Acts as a Negative Regulator of Osteoclastogenesis by Modulating the Acid-Sensing OGR1/NFAT Signaling Pathway? J. Bone Miner. Res. 22, 1612-1620 (2007) (Neutralization)


A115M P110, (mitochondrial membrane protein)
1. Criollo, A., et al, 'Regulation of autophagy by the inositol trisphosphate receptor' Cell Death and Differentiation 2007, 14, , 1029-1039


c155m p27
1. Vella, V., et al, 'Interleukin-4 stimulates papillary thyroid cancer cell survival: Implications in patients with thyroid cancer and concomitant Graves' disease' Journal of Clinical Endocrinol. Metab 2004, 89, , 2880-2889


X1017 Phosphotyrosine FITC conjugate
1. Tahiri, A., et al. ?Differential Inhibition of Ex-Vivo Tumor Kinase Activity by Vemurafenib in BRAF(V600E) and BRAF Wild-Type Metastatic Malignant Melanoma.? PLoS One, 8, e72692 (2013)
2. Galvani, E., et al. ?Molecular Mechanisms Underlying the Antitumor Activity of 3-Aminopropanamide Irreversible Inhibitors of the Epidermal Growth Factor Receptor in Non-Small Cell Lung Cancer.? Neoplasia, 15, 61-72 (2013)


X1017S phosphotyrosine FITC conjugate
1. Tahiri, A., et al. ?Differential Inhibition of Ex-Vivo Tumor Kinase Activity by Vemurafenib in BRAF(V600E) and BRAF Wild-Type Metastatic Malignant Melanoma.? PLoS One, 8, e72692 (2013)
2. Galvani, E., et al. ?Molecular Mechanisms Underlying the Antitumor Activity of 3-Aminopropanamide Irreversible Inhibitors of the Epidermal Growth Factor Receptor in Non-Small Cell Lung Cancer.? Neoplasia, 15, 61-72 (2013)


X1021 phosphotyrosine unconjugated
1. Welham, N.V., et al. ?Proteomic analysis of a decellularized human vocal fold mucosa scaffold using 2D electrophoresis and high-resolution mass spectrometry.? Biomaterial, Jan 2013, 34(4): 669-676.


X1155P PIG3 (p53 inducible gene 3)
1. Marusyk, A., et al, 'p53 mediates senescence-like arrest induced by chronic replicational stress' Molecular abd Cell Biology 2007, 27, , 5336-5351


X1156M PIG3 (p53 inducible gene 3)
1. Marusyk, A., et al, 'p53 mediates senescence-like arrest induced by chronic replicational stress' Molecular abd Cell Biology 2007, 27, , 5336-5351


X1491M Prostaglandin-E2 receptor EP2
1. Nahid Waleh, et al., 'Prostaglandin E2-Mediated Relaxation of the Ductus Arteriosus:Effects of Gestational Age o g Protein-Coupled Receptor Expression, Signaling & Vasomotor Control' Circulation 2004, 110, , 2326-2332


X1192 Protein A Agarose
1. Abdullah, M., et al, 'Killing of dsrA Mutants of Haemophilus ducreyi by Normal Human Serum Occurs via the Classical Complement Pathway and Is Initiated by Immunoglobulin M Binding' Infectious Immunology 2005, 73, , 3431-3439


p120p Protein Phosphatase 1 gamma 1
1. Maeda, T., et al, 'Involvement of serine/threonine protein phosphatases sensitive to okadaic acid in restraint stress-induced hyperlocomotion in cocaine-sensitized mice' British Journal of Pharmacology 2006, 148, , 405-412


p220p Protein Phosphatase V/C (PP6C)
1. Leung-Pineda, V., et a, 'Phosphorylation of Chk1 by ATR Is Antagonized by a Chk1-Regulated Protein Phosphatase 2A Circuit' Molecular Cell Biology 2006, 26, , 7529-7538


p240p PTP (HCPTP)
1. Chen, Z., et al, 'Loss of Zap-70 and low molecular weight phosphotyrosine phosphatase occurs after therapy in a patient with B-chronic lymphocytic leukemia' Leukemia 2005, 19, , 1503-1505
2. Lee, C.F., et al. ?Regulation of Monocyte Adhesion and Migration by Nox4.? PLoS One, 8, e66964 (2013)


RG14 Rat anti-mouse IgG1k PE
1. Anderson, S.J., et al, 'The cytoplasmic domain of CD4 is sufficient for its down-regulation from the cell surface by human immunodeficiency virus type 1 Nef' Journal of Virology 1994, 68, , 3092-3101


X1024 SNAP
1. Chae, T., et al, 'The hyh mutation uncovers roles for Snap in apical protein localization and control of neural cell fate' Nature Geneetics 2004, 36, , 264-270


X1598M SNAP25
1. Wang, L., et al. ? ?SNAP inhibits AMPK signaling to reduce mitochondrial biogenesis and dephosphorylates Thr172 in AMPK in vitro.? Nat. Commun., 4, 1559 (2013)


X1867P SPARC
1. Kelly, Kimberly A. et al, 'SPARC is a VCAM-1 counter-ligand that mediates leukocyte transmigration' Journal of Leukocyte Biology 2007, 81, 3, 748-756


X2059P Sphingomyelin Synthase 2
1. Burns, T.A., et al. ?Sphingomyelin synthase 1 activity is regulated by BCR-ABL oncogene.? Journal of Lipid Research, Mar 2013, 54(3): 794-805


X1093P Sphingosine 1 Phosphate Receptor 1 (EDG-1) CT
1) Western Blot: 'Transduction of Multiple Effects of Sphingosine 1-Phosphate (S1P) on T Cell Function by the S1P1 G Protein Coupled Receptor.' Dorsam, Glenn, et al., Journal of Immunology, 2003, 3500-3507.
2) Immunocytochemistry: 'Differential transactivation of Sphingoise-1 Phosphate Receptors modulate NGF-induced neurite extension.' Toman, Rachelle E., et al., Journal of Cell Biology, Aug. 2, 2004, vol 166, no.3, 381-392.
3) Western Blot: 'Transactivation of Sphingosine-1-Phosphate Recpetors by FceRI Triggering is Required for Normal Mast Cell Degranulation and Chemotaxis.' Jolly, Puneet S., et al., Journal of Experimental Medicine, April 5, 2004, vol. 199, no. 7, 959-970.
4) Western Blot: 'Induction of pro-angiogenic signaling by a synthetic peptide derived from the second intracellular loop of S1P3 (EDG3).' Blood, 2003, 102, 2099-2107.


X1094P Sphingosine 1 Phosphate Receptor 5 CT (EDG-8)
1. Toman, Rachelle E. et al, 'Differential transactivation of sphingosine-1-phosphate receptors modulates NGF-induced neurite extesion' The Journal of Cell Biology 2004, 166, 3, 381-392


c176m Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT
1. Ishii, Isao, et al., 'Selective Loss of Sphingosine 1-Phosphate Signaling with No Obvious Phentoypic Abnormality in Mice Lacking Its G Protein-coupled Receptor, LPb3/EDG3' The Journal of Biogical Chemistry 2001, 276, 36, 33697-33704
2. Coussin, Frederic, et al, 'Comparison of Sphingosine 1-Phosphate-Induced Intracellular Signaling Pathways in Vascular Smooth Muscles' Circulation Research 2002, 91, , 151-157
3. Sun, X., et al. ?Sphingosine-1-Phosphate Receptor-3 Is a Novel Biomarker in Acute Lung Injury.? American Journal of Respiratory Cell and Molecular Biology, Nov 2012, 47(5): 628-636.


X1589P Sphingosine 1 Phosphate 3 Receptor (EDG-3) CT
1. Sun, X., et al. ?Sphingosine-1-Phosphate Receptor-3 Is a Novel Biomarker in Acute Lung Injury.? American Journal of Respiratory Cell and Molecular Biology, Nov 2012, 47(5): 628-636.


X1590P Sphingosine 1 Phosphate 3 Receptor (EDG-3) NT
1. Licht, Tamar et al, 'Induction of pro-angiogenic signaling by a synthetic peptide derived from the scond intracellular loop of SIP3 (EDG3)' Blood 2003, 102, 6, 2099-2107
2. Sun, X., et al. ?Sphingosine-1-Phosphate Receptor-3 Is a Novel Biomarker in Acute Lung Injury.? American Journal of Respiratory Cell and Molecular Biology, Nov 2012, 47(5): 628-636.


X1182P Sphingosine 1 Phosphate 4 Receptor (CT) (EDG6)
1. Dorsam, Glenn et al, 'Transducion of Multiple Effects of Sphingosine 1-Phosphate (S1P) on T Cell Function by the S1P1 G Protein-Coupled Receptor
' The Journal of Immunology 2003, 171, 7, 3500-3507


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