Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

References

X1592P Sphingosine 1 Phosphate 4 Receptor (IN) (EDG6)
1. Ohotski, J., et al. ? Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer.? British Journal of Cancer, Apr 2012, 106(8): 1453-1459.


X2717B Sphingosine 1 Phosphate 4 Receptor (NT) (EDG6)
1. Ohotski, J., et al. ? Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer.? British Journal of Cancer, Apr 2012, 106(8): 1453-1459.


X1593P Sphingosine 1 Phosphate 4 Receptor (NT) (EDG6) NT
1. Ohotski, J., et al. ? Expression of sphingosine 1-phosphate receptor 4 and sphingosine kinase 1 is associated with outcome in oestrogen receptor-negative breast cancer.? British Journal of Cancer, Apr 2012, 106(8): 1453-1459.


X1631P Sphingosine 1-Phosphate Lyase 1
1. Katsuma, Susumu, et al, 'Signalling mechanisms in sphingosine 1-phosphate-promoted mesangial cell proliferation ' Genes to Cells 2002, 7, , 1217-1230


X1627P Sphingosine Kinase 1
1. Manikandan, Jayapal, et al, 'Genome-wide gene expression profiling of human mast cells stimulated by IgE or Fc RI-aggregation reveals a complex network of genes involved in inflammatory responses ' BMC Genomics 2006, 7, 210, Online only
2. Yang, Jianfei, et al., 'Sphingosine Kinase 1 Is a Negative Regulator of CD4 Th1 Cells ' The Journal of Immunology 2005, 175, , 6580-6588
3.El-Shewy, H.M., et al. ?Low-Density Lipoprotein Induced Expression of Connective Tissue Growth Factor via Transactivation of Sphingosine 1-Phosphate Receptors in Mesangial Cells.? Molecular Endocrinology, May 2012, 26(5): 833-845.


X1849P Sphingosine Kinase 1,(pS225), Mouse Reactive
1. El-Shewy, H.M., et al. ?Low-Density Lipoprotein Induced Expression of Connective Tissue Growth Factor via Transactivation of Sphingosine 1-Phosphate Receptors in Mesangial Cells.? Molecular Endocrinology, May 2012, 26(5): 833-845.


X1072M TCF-1
1. Dorfman, David M. et al., 'Loss of Expression of the WNT/Beta Catenin Signaling Pathway Transcription Factors Lymphoid Enhancer Factor-1 (LEF-1) and T Cell factor-1 (TCF-10 in a Subset of Peripheral T Cel Lymphomas' The American Journal of Pathology 2003, 162, , 1539-1544
2. Tobimatsu, Takako, et al, 'Parathyroid Hormone Increaes Beta Catenin levels through Smad3 in Mouse Osteblastic Cells' Endocrinology 2006, 147, 5, 2583-2590


X1070M TCF-4
1. Sampson, Ellen, M., et al, 'Negative regulation of the Wnt- Beta-Catenin pathway by the transcriptional represor HBPQ' The EMBO Journal 2001, 20, , 4500-4511
2. Uematsu, Kazutsugu, et al, 'Wnt Pathway Activation in Mesothelioma: Evidence of Dishevelled Overexpression and Transcriptional Activity of Beta Catenin' Journal of Cancer Research 2003, 63, , 4547-4551
3. Shimura, Tatsuo, 'Galectin-3, a Novel Binding Partner of Beta Catenin' Cancer Research 2004, 64, , 6363-6367


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