Western Blotting Protocol

Western Blotting Protocol Lysate preparation Lysate preparation will depend of the source of cells (Monolayer, Cell suspension or Tissue samples). In general, Cells are washed with cold 1xPBS, subsequently lysed with an appropriate buffer (i.e. RIPA buffer) containing freshly added protease inhibitors. Passed through a 21G needle to shear the DNA and then centrifuged at 10K rpm for 10min at … Continue reading Western Blotting Protocol