Sandwich Enzyme Immuno-Assay (EIA) for analyte detection and/or quantitation

Sandwich Enzyme Immuno-Assay (EIA) for analyte detection and/or quantitation Background Sandwich ELISA’s are the tool of choice for measuring small quantities of antigen in complex biological mixtures. The principle involves ‘capturing’ an antigen/analyte between two ‘layers’ of antibodies which target separate epitopes on the target molecule (i.e. capture and detection antibody). The target molecule must of necessity contain more than … Continue reading Sandwich Enzyme Immuno-Assay (EIA) for analyte detection and/or quantitation