Immunoprecipitation Protocol

Immunoprecipitation Protocol Wash Buffer 20 mM Tris, pH 8.0 containing 0.2% Trition X100 Elution Buffer Wash buffer containing 4% SDS (and also include protease inhibitors if required) NOTE: The choice of wash and elution buffers should be empirically determined. The buffers listed here are for general use and will not be ideal for all proteins/systems. If phospho proteins are being … Continue reading Immunoprecipitation Protocol