Exalpha Biologicals, Inc.

Latest News

Exalpha Biologicals, Inc. is now a US distributor for Nordic-Mubio products

Nordic-MUbio develops and manufactures high quality antibody reagents for use in R&D. All our products are manufactured according to strict ISO 9001 quality guidelines, and our focus is on developing antibodies targetting Cell Adhesion, Nuclear and Cytoskeletal Proteins.

Nordic-MUbio is one of the leading antibody companies developing antibodies for zebrafish research – we currently have over 50 antibodies validated for immunofluorescence in zebrafish.

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Latest References

Akazawa, Y., et al. "Endoplasmic Reticulum Stress Contributes to Helicobacter Pylori VacA-Induced Apoptosis" PLoS ONE 8(12): e82322

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Homa, N.J., et al. "Epstein-Barr Virus Induces Global Changes in Cellular mRNA Isoform Usage That Are Important for the Maintenance of Latency" J. Virol., 2013, 87, 12291-12301

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Helpful Hints for Using Exalpha Immunofluorescence Kits

Issue

Tip


Autofluorescence

  1. One method of checking for autofluorescence is to look at the slide with multiple filters. If the same signal is seen with multiple filters, it could be autofluorescence.
  2. To help decrease autofluorescence, add a Sudan Black step after the SA-FL incubation.
    1. Make up the Sudan Black at 0.2% in 70% Ethanol (mix for 2 hours in the dark).
    2. After the SA-FL incubation and wash step, add 100 µL of 0.2% Sudan Black to the slide.
    3. Incubate for 15 minutes at room temperature in the dark.
    4. Wash 8 X 1 minute using the wash buffer recommended in the assay specific protocol.
    5. Mount and view the slide under the microscope.

Supressed Signal

If Sudan Black was used:
  1. While the use of Sudan Black can help decrease autofluorescence, it can also decrease signal. Sudan Black should be used with caution.
  2. The addition of 0.02% tween to the wash step following the Sudan Black incubation may help to increase the signal.
  3. Sudan Black should be made up fresh each time.
  4. Sudan Black can be tested at at a lower concentration (e.g., 0.025%).
Additional tips:
  1. Completed slides should be stored in the dark when they are not in use.
  2. Slides should be analyzed shortly after the mounting media is dry.

The signal is
difficult to find

  1. Use the bright field setting on the microscope to focus in on the correct section of the tissue prior to using the excitation wavelength listed in the kit specific protocol.
  2. Make sure that the work area surrounding the microscope is dark.
  3. Allow your eyes to adjust to the dark prior to viewing the slides.