Exalpha Biologicals, Inc.

Accelerating the Pace of Discovery

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.


Two more of our excellent products have been published by PubMed:

Potential actionable targets in appendiceal cancer detected by immunohistochemistry, fluorescent in situ hybridization, and mutational analysis
Borazanci, E., et al., J. Gastrointest. Oncol., 8, 164-172 (2017)
Using Exalpha SPARC Antibody (Cat. No. X1867P)

Molecular mechanism underlying the pharmacological interactions of the protein kinase C-β inhibitor enzastaurin and erlotinib in non-small cell lung cancer cells
Steen, N.V., et al., Am. J. Cancer Res., 7, 816-830 (2017)
Using Exalpha's FITC labeled anti PY20 Antibody (Cat. No. X1017)

Buffer Formulations

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  • Acid precipitation solution for precipitation of nucleic acids
  • Ammonium Sulfate, Saturated (SAS)
  • Ammonium Acetate, 10M
  • ATP, 100mM
  • Carbonate buffer
  • Church's Buffer for Northern and Southern Blotting Hybridization
  • Calcium- and magnesium-free Dulbeccos phosphate-buffered saline (CMF-DPBS)
  • DTT (dithiothreitol), 1 M
  • EDTA (ethylenediaminetetraacetic acid), 0.5 M (pH 8.0)
  • Eisen Buffer (10x PBS) (10 Liters)
  • HBSS (Hanks balanced salt solution)
  • HEPES-buffered saline solution, 2× solution
  • Lysogeny broth (LB) Medium
  • Phosphate buffer, pH 7.2
  • Phosphate-buffered saline (PBS) (1x)
  • Phosphate-buffered saline (PBS) (10x)
  • Potassium acetate buffer, 0.1 M
  • Potassium phosphate buffer, 0.1 M
  • SDS electrophoresis buffer, 5× solution
  • SDS sample buffer
  • TAE (Tris/acetate/EDTA) electrophoresis buffer, 10× solution
  • TBE (Tris/borate/EDTA) electrophoresis buffer, 10× solution
  • TBS (Tris-buffered saline)
  • TE (Tris/EDTA) buffer
  • TEA (triethanolamine) solution