Frontier™ ApoMark DNA Fragmentation Detection Kit
Exalpha's Frontier™ ApoMark™ DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic cell nuclei in paraffin-embedded tissue sections, tissue cryosections and in cell preparations fixed on slides. Available using DAB substrate for visualization as well as fluroescent conjugates for immunofluorescence assays
- All reagents in dropper bottles prediluted and ready to use
- All buffers and enzymes necessary for staining included
- Can be used on tissue from any species
- Streptavidin-Biotin detection system
ApoMark™ kit (X2044K) using paraffin fixed human tonsil tissue, 10 μm sections (1000X). A] Section processed and counter-stained with methyl green according to the ApoMark™ manual. B] Counter-stain step was eliminated to more clearly illustrate the level of positive staining in the germinal centers of tonsil tissue. C] Section treated with DNase I in order to generate a positive control slide. Note all nuclei stain positive. The use of DNase I generates free 3’-OH groups on cellular DNA, these free 3’-OH groups are then labeled with biotin-nucleotide by the TdT in the ApoMark™ kit. D] Negative control, the TdT enzyme step was eliminated thereby generating a negative slide.