Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

News

Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Frontier™ ApoMark DNA Fragmentation Detection Kit

Exalpha's Frontier™ ApoMark™ DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic cell nuclei in paraffin-embedded tissue sections, tissue cryosections and in cell preparations fixed on slides. Available using DAB substrate for visualization as well as fluroescent conjugates for immunofluorescence assays


Features

  • All reagents in dropper bottles prediluted and ready to use
  • All buffers and enzymes necessary for staining included
  • Can be used on tissue from any species
  • Streptavidin-Biotin detection system

Ordering Information

  • X2044K1 - 30 tests
  • X2044K2 - 60 tests
  • For larger quantity pricing, please contact customer service.

Legend:
ApoMark™ kit (X2044K) using paraffin fixed human tonsil tissue, 10 μm sections (1000X). A] Section processed and counter-stained with methyl green according to the ApoMark™ manual. B] Counter-stain step was eliminated to more clearly illustrate the level of positive staining in the germinal centers of tonsil tissue. C] Section treated with DNase I in order to generate a positive control slide. Note all nuclei stain positive. The use of DNase I generates free 3’-OH groups on cellular DNA, these free 3’-OH groups are then labeled with biotin-nucleotide by the TdT in the ApoMark™ kit. D] Negative control, the TdT enzyme step was eliminated thereby generating a negative slide.