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Sheep anti Human PTP (HCPTP)

$268.00

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SKU: P240P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
"CHO cells were transfected with either Vector (pSRa) or expression 
vector driving expression of HCPTPA (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2mg of lysate protein was incubated for 1 h with 10ml of sheep-anti-LMWPTP containing anti-serum, and immuno-precipitated proteins recovered by Protein A/G were separated on PAGE (12%), and HCPTPA detected using a mouse anti-sheep monoclonal antibody (Cat # X1206M)."

"CHO cells were transfected with either Vector (pSRa) or expression vector driving expression of HCPTPA (pSRa-LMWPTP), and harvested in Triton X-100 lysis buffer at 72 h after transfection. 2mg of lysate protein was incubated for 1 h with 10ml of sheep-anti-LMWPTP containing anti-serum, and immuno-precipitated proteins recovered by Protein A/G were separated on PAGE (12%), and HCPTPA detected using a mouse anti-sheep monoclonal antibody (Cat # X1206M)."