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Sheep anti Human ELK rec tyr Kinase (Eph B1 Cy.)

$268.00

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SKU: S110P Categories : Primary antibodies, Primary antibodies, Polyclonal Antibody
"Legend: CHO cells were trasfected with pSR-α huEphB1/HA, driving expression of HA epitope tagged EphB1.  Cell lysates (200-300µg protein) were immunoprecipitated using 5 µl rabbit-anti-sheep antibody.  Immunocomplexes were recovered on Protein A/G-Sepharose, separated by SDS-PAGE and EphB1/HA protein was detected by immunoblot using an anti-HA epitope antibody (Cat. No. X1000), and an anti-mouse IgG/peroxidase conjugate.  Sheep were immunized with the following GST fusion proteins from EphB1 (huELK) sequences:
Cy:  aa 566-984
SAM: aa 883-984
EXT: aa 16-351"

"Legend: CHO cells were trasfected with pSR-α huEphB1/HA, driving expression of HA epitope tagged EphB1. Cell lysates (200-300µg protein) were immunoprecipitated using 5 µl rabbit-anti-sheep antibody. Immunocomplexes were recovered on Protein A/G-Sepharose, separated by SDS-PAGE and EphB1/HA protein was detected by immunoblot using an anti-HA epitope antibody (Cat. No. X1000), and an anti-mouse IgG/peroxidase conjugate. Sheep were immunized with the following GST fusion proteins from EphB1 (huELK) sequences: Cy: aa 566-984 SAM: aa 883-984 EXT: aa 16-351"