Secondary antibodies are valuable biological tools that are employed extensively in research applications and diagnostic assays. Secondary antibodies are conjugated to reporter molecules, such as enzymes or fluorochromes, which provide a signal that can be detected and measured. They are commonly used in antibody based applications to detect an antigen specific primary antibody that is not already conjugated to a reporter. Applications that may require a secondary antibody include immunohistology, immunofluorescence, western blotting, enzyme linked immunosorbent assays (ELISA), flow cytometry and lateral flow assays. Careful selection of suitable secondary antibodies also allows researchers to perform simultaneous labelling of multiple targets in a single sample.

A diverse range of monoclonal (Mab), polyclonal (PAb) secondary antibodies are available that vary according to the host, in which the antibody was raised. They are also grouped according to characteristics of the primary antibody recognised with respect to animal species, antibody class and region of the antibody. Secondary antibodies may also be supplied as whole antibodies or as antibody fragments, which can provide technical advantages in some applications.

In addition, antibodies can be conjugated to numerous different reporter molecules thus providing researchers and assay developers with enormous application and assay design flexibility. The correct selection of an appropriate secondary antibody is vital to the success of any immunostaining technique.

Exalpha manufacture and supply polyclonal secondary antibodies that have been raised in goats and are specific for the Fc portion of mouse IgG. The secondary antibodies recognise all subclasses of mouse IgG and are available conjugated to fluorescein isothiocyanate (FITC) or tetramethylrhodamine isothiocyanate (TRITC). The antibodies are suitable for use in immunocytochemistry, immunofluorescence, immunohistochemistry and ELISA applications.

In addition, Exalpha offers an extensive range of high quality polyclonal secondary antibodies that are suitable for the detection of mouse and rat immunoglobulin classes and subclasses. The range includes secondary antibodies conjugated to horseradish peroxidase, biotin, FITC or TRITC. The antibodies have been tested for Ig cross reactivity and are designed for use in a wide range of research applications.

Code Name Product Type Clone
ZU105 Anti-Goat IgG, biotinylated
ZU102 Anti-Mouse IgG, biotinylated
ZU122 Anti-Mouse IgG, biotinylated (Rat absorbed)
ZU114 Anti-Mouse/Rabbit IgG, biotinylated
ZU101 Anti-Rabbit IgG, biotinylated
ZU124 Anti-Rat IgG, biotinylated (Mouse absorbed)
DoAG/IgG(H+L) Donkey anti Goat IgG (heavy and light chains) Polyclonal
DoAG/IgG(H+L)/7S Donkey anti Goat IgG (heavy and light chains) Polyclonal
DoAG/IgG(H+L)/Bio Donkey anti Goat IgG (heavy and light chains), conjugated with Biotin Polyclonal
DoAG/IgG(H+L)/FITC Donkey anti Goat IgG (heavy and light chains), conjugated with FITC Polyclonal
DoAG/IgG(H+L)/PO Donkey anti Goat IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
DoAG/IgG(H+L)/TRITC Donkey anti Goat IgG (heavy and light chains), conjugated with TRITC Polyclonal
FITC/1 Fluorescein Isothiocynate Isomer 1
GAB/IgG(Fc) Goat anti Bovine IgG (Fc specific) Polyclonal
GAB/IgG(H+L) Goat anti Bovine IgG (heavy and light chains) Polyclonal
GAB/IgG(H+L)/7S Goat anti Bovine IgG (heavy and light chains) Polyclonal
GAB/IgG(H+L)/Bio Goat anti Bovine IgG (heavy and light chains), conjugated with Biotin Polyclonal
GAB/IgG(H+L)/FITC Goat anti Bovine IgG (heavy and light chains), conjugated with FITC Polyclonal
GAB/IgG(H+L)/PO Goat anti Bovine IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
GAB/TSP/FITC Goat anti Bovine Serum proteins, conjugated with FITC Polyclonal
GA/ConA/PO Goat anti Canavalia Concanavalin A, conjugated with Horseradish peroxidase Polyclonal
GACa/IgA(Fc) Goat anti Cat IgA (Fc specific) Polyclonal
GACa/IgG(H+L) Goat anti Cat IgG (heavy and light chains) Polyclonal
GACa/Ig Goat anti Cat IgG IgA IgM (heavy and light chains) Polyclonal
GACa/IgM(Fc) Goat anti Cat IgM (Fc specific) Polyclonal
GACh/IgA(Fc) Goat anti Chicken IgA (Fc specific) Polyclonal
GACh/IgA(Fc)/7S Goat anti Chicken IgA (Fc specific) Polyclonal
GACh/IgA(Fc)/Bio Goat anti Chicken IgA (Fc specific), conjugated with Biotin Polyclonal
GACh/IgA(Fc)/FITC Goat anti Chicken IgA (Fc specific), conjugated with FITC Polyclonal
GACh/IgA(Fc)/PO Goat anti Chicken IgA (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GACh/IgG(Fc) Goat anti Chicken IgG (Fc specific) Polyclonal
GACh/Fab Goat anti Chicken IgG Fab Polyclonal
GaCh/Ig Goat anti Chicken IgG IgA IgM (heavy and light chains) Polyclonal
GACh/IgM(Fc) Goat anti Chicken IgM (Fc specific) Polyclonal
GACh/IgM(Fc)/7S Goat anti Chicken IgM (Fc specific) Polyclonal
GACh/IgM(Fc)/Bio Goat anti Chicken IgM (Fc specific), conjugated with Biotin Polyclonal
GACh/IgM(Fc)/FITC Goat anti Chicken IgM (Fc specific), conjugated with FITC Polyclonal
GACh/IgM(Fc)/PO Goat anti Chicken IgM (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GAD/IgA(Fc) Goat anti Dog IgA (Fc specific) Polyclonal
GAD/IgA(Fc)/7S Goat anti Dog IgA (Fc specific) Polyclonal
GAD/IgA(Fc)/Bio Goat anti Dog IgA (Fc specific), conjugated with Biotin Polyclonal
GAD/IgA(Fc)/FITC Goat anti Dog IgA (Fc specific), conjugated with FITC Polyclonal
GAD/IgA(Fc)/PO Goat anti Dog IgA (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GAD/IgG(Fc) Goat anti Dog IgG (Fc specific) Polyclonal
GAD/IgG(Fc)/7S Goat anti Dog IgG (Fc specific) Polyclonal
GAD/IgG(Fc)/Bio Goat anti Dog IgG (Fc specific), conjugated with Biotin Polyclonal
GAD/IgG(Fc)/FITC Goat anti Dog IgG (Fc specific), conjugated with FITC Polyclonal
GAD/IgG(Fc)/PO Goat anti Dog IgG (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GAD/IgG(H+L) Goat anti Dog IgG (heavy and light chains) Polyclonal
GAD/IgG(H+L)/7S Goat anti Dog IgG (heavy and light chains) Polyclonal
GAD/IgG(H+L)/Bio Goat anti Dog IgG (heavy and light chains), conjugated with Biotin Polyclonal
GAD/IgG(H+L)/FITC Goat anti Dog IgG (heavy and light chains), conjugated with FITC Polyclonal
GAD/IgG(H+L)/PO Goat anti Dog IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
GAD/IgM(Fc) Goat anti Dog IgM (Fc specific) Polyclonal
GAD/IgM(Fc)/7S Goat anti Dog IgM (Fc specific) Polyclonal
GAD/IgM(Fc)/Bio Goat anti Dog IgM (Fc specific), conjugated with Biotin Polyclonal
GAD/IgM(Fc)/FITC Goat anti Dog IgM (Fc specific), conjugated with FITC Polyclonal
GAD/IgM(Fc)/PO Goat anti Dog IgM (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GADu/Ig Goat anti Duck IgG IgA IgM (heavy and light chains) Polyclonal
GADu/IgM(Fc) Goat anti Duck IgM (Fc specific) Polyclonal
GADu/IgM(Fc)/7S Goat anti Duck IgM (Fc specific) Polyclonal
GADu/IgM(Fc)/Bio Goat anti Duck IgM (Fc specific), conjugated with Biotin Polyclonal
GADu/IgM(Fc)/FITC Goat anti Duck IgM (Fc specific), conjugated with FITC Polyclonal
GADu/IgM(Fc)/PO Goat anti Duck IgM (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GAGp/IgG(Fc) Goat anti Guinea Pig IgG (Fc specific) Polyclonal
GAGp/IgG(H+L) Goat anti Guinea Pig IgG (heavy and light chains) Polyclonal
GAGp/IgG(H+L)/7S Goat anti Guinea Pig IgG (heavy and light chains) Polyclonal
GAGp/IgG(H+L)/Bio Goat anti Guinea Pig IgG (heavy and light chains), conjugated with Biotin Polyclonal
GAGp/IgG(H+L)/FITC Goat anti Guinea Pig IgG (heavy and light chains), conjugated with FITC Polyclonal
GAGp/IgG(H+L)/PO Goat anti Guinea Pig IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
GAGp/IgG1 Goat anti Guinea Pig IgG (subclass specific) Polyclonal
GAGp/IgG1/Bio Goat anti Guinea Pig IgG (subclass specific), conjugated with Biotin Polyclonal
GAGp/IgG1/FITC Goat anti Guinea Pig IgG (subclass specific), conjugated with FITC Polyclonal
GAGp/IgG1/PO Goat anti Guinea Pig IgG (subclass specific), conjugated with Horseradish peroxidase Polyclonal
GAGp/Fab Goat anti Guinea Pig IgG Fab Polyclonal
GAGp/Fab/FITC Goat anti Guinea Pig IgG Fab, conjugated with FITC Polyclonal
GAGp/IgG2 Goat anti Guinea Pig IgG2 (subclass specific) Polyclonal
GAGp/IgG2/Bio Goat anti Guinea Pig IgG2 (subclass specific), conjugated with Biotin Polyclonal
GAGp/IgG2/FITC Goat anti Guinea Pig IgG2 (subclass specific), conjugated with FITC Polyclonal
GAGp/IgG2/PO Goat anti Guinea Pig IgG2 (subclass specific), conjugated with Horseradish peroxidase Polyclonal
GAGp/IgM(Fc)/Bio Goat anti Guinea Pig IgM (Fc specific), conjugated with Biotin Polyclonal
GAGp/IgM(Fc)/FITC Goat anti Guinea Pig IgM (Fc specific), conjugated with FITC Polyclonal
GAGp/IgM(Fc)/PO Goat anti Guinea Pig IgM (Fc specific), conjugated with Horseradish peroxidase Polyclonal
GAHa/IgG(H+L) Goat anti Hamster IgG (heavy and light chains) Polyclonal
GAHo/IgG(Fc) Goat anti Horse IgG (Fc specific) Polyclonal
GAHo/IgG1(T) Goat anti Horse IgG1(T) Polyclonal
GAHo/IgM(Fc) Goat anti Horse IgM (Fc specific) Polyclonal
GAHu/BJK(SD+HD) Goat anti Human Ig kappa light chain (free and bound) Polyclonal
GAHu/BJK(SD+HD)/7S Goat anti Human Ig kappa light chain (free and bound) Polyclonal
GAHu/BJK/Ifix Goat anti human Ig kappa light chain (free and bound) Polyclonal
GAHu/BJK(SD+HD)/FITC Goat anti Human Ig kappa light chain (free and bound), conjugated with FITC Polyclonal
GAHu/BJK(SD+HD)/TRITC Goat anti Human Ig kappa light chain (free and bound), conjugated with TRITC Polyclonal
SR/GAHu/BJK(SD+HD)/7S Goat anti Human Ig kappa light chains (free and bound) Polyclonal
SR/GAHu/BJK(SD+HD)/Bio Goat anti Human Ig kappa light chains (free and bound), conjugated with Biotin Polyclonal
SR/GAHu/BJK(SD+HD)/FITC Goat anti Human Ig kappa light chains (free and bound), conjugated with FITC Polyclonal
SR/GAHu/BJK(SD+HD)/PO Goat anti Human Ig kappa light chains (free and bound), conjugated with Horseradish peroxidase Polyclonal
SR/GAHu/BJK(SD+HD)/TRITC Goat anti Human Ig kappa light chains (free and bound), conjugated with TRITC Polyclonal
GAHu/BJL(SD+HD) Goat anti Human Ig lambda light chain (free and bound) Polyclonal
GAHu/BJL(SD+HD)/7S Goat anti Human Ig lambda light chain (free and bound) Polyclonal
GAHu/BJL(SD+HD)/FITC Goat anti Human Ig lambda light chain (free and bound), conjugated with FITC Polyclonal
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