Secondary antibodies are valuable biological tools that are employed extensively in research applications and diagnostic assays. Secondary antibodies are conjugated to reporter molecules, such as enzymes or fluorochromes, which provide a signal that can be detected and measured. They are commonly used in antibody based applications to detect an antigen specific primary antibody that is not already conjugated to a reporter. Applications that may require a secondary antibody include immunohistology, immunofluorescence, western blotting, enzyme linked immunosorbent assays (ELISA), flow cytometry and lateral flow assays. Careful selection of suitable secondary antibodies also allows researchers to perform simultaneous labelling of multiple targets in a single sample.

A diverse range of monoclonal (Mab), polyclonal (PAb) secondary antibodies are available that vary according to the host, in which the antibody was raised. They are also grouped according to characteristics of the primary antibody recognised with respect to animal species, antibody class and region of the antibody. Secondary antibodies may also be supplied as whole antibodies or as antibody fragments, which can provide technical advantages in some applications.

In addition, antibodies can be conjugated to numerous different reporter molecules thus providing researchers and assay developers with enormous application and assay design flexibility. The correct selection of an appropriate secondary antibody is vital to the success of any immunostaining technique.

Exalpha manufacture and supply polyclonal secondary antibodies that have been raised in goats and are specific for the Fc portion of mouse IgG. The secondary antibodies recognise all subclasses of mouse IgG and are available conjugated to fluorescein isothiocyanate (FITC) or tetramethylrhodamine isothiocyanate (TRITC). The antibodies are suitable for use in immunocytochemistry, immunofluorescence, immunohistochemistry and ELISA applications.

In addition, Exalpha offers an extensive range of high quality polyclonal secondary antibodies that are suitable for the detection of mouse and rat immunoglobulin classes and subclasses. The range includes secondary antibodies conjugated to horseradish peroxidase, biotin, FITC or TRITC. The antibodies have been tested for Ig cross reactivity and are designed for use in a wide range of research applications.

Code Name Product Type Clone
ZU105 Anti-Goat IgG, biotinylated
ZU102 Anti-Mouse IgG, biotinylated
ZU122 Anti-Mouse IgG, biotinylated (Rat absorbed)
ZU114 Anti-Mouse/Rabbit IgG, biotinylated
ZU101 Anti-Rabbit IgG, biotinylated
ZU124 Anti-Rat IgG, biotinylated (Mouse absorbed)
ABIG Chicken anti Bovine IgG (H+L) Secondary antibodies
ABIG-AGA-1 Chicken anti Bovine IgG (H+L), conjugated with Agarose Secondary antibodies
ABIG-AGA-2 Chicken anti Bovine IgG (H+L), conjugated with Agarose Secondary antibodies
ABIG-AGA-5 Chicken anti Bovine IgG (H+L), conjugated with Agarose Secondary antibodies
ABIG-B Chicken anti Bovine IgG (H+L), conjugated with Biotin Secondary antibodies
ABIG-F Chicken anti Bovine IgG (H+L), conjugated with FITC Secondary antibodies
ABIG-HRP Chicken anti Bovine IgG (H+L), conjugated with HRP Secondary antibodies
ABIG-10 Chicken anti Bovine IgG (H+L), not affiinity purified Secondary antibodies
ACerIG Chicken anti Cervid IgG (H+L) Secondary antibodies
ACerIG-B Chicken anti Cervid IgG (H+L), conjugated with Biotin Secondary antibodies
ACerIG-F Chicken anti Cervid IgG (H+L), conjugated with FITC Secondary antibodies
ACerIG-HRP Chicken anti Cervid IgG (H+L), conjugated with HRP Secondary antibodies
ACRIG Chicken anti Cotton Rat IgG (H+L) Secondary antibodies
ACRIG-HRP Chicken anti Cotton Rat IgG (H+L), conjugated with HRP Secondary antibodies
ADIG Chicken anti Dog IgG (H+L) Secondary antibodies
ADIG-B Chicken anti Dog IgG (H+L), conjugated with Biotin Secondary antibodies
ADIG-F Chicken anti Dog IgG (H+L), conjugated with FITC Secondary antibodies
ADIG-HRP Chicken anti Dog IgG (H+L), conjugated with HRP Secondary antibodies
AHoIG-B Chicken anti Horse IgG (H+L), conjugated with Biotin Secondary antibodies
AHIgGFab Chicken anti Human IgG (Fab)2 Secondary antibodies
AHIgGFab-B Chicken anti Human IgG (Fab)2, conjugated with Biotin Secondary antibodies
AHIgGFab-F Chicken anti Human IgG (Fab)2, conjugated with FITC Secondary antibodies
AHIgGFab-HRP Chicken anti Human IgG (Fab)2, conjugated with HRP Secondary antibodies
AHIgG Chicken anti Human IgG (H/L) Secondary antibodies
AHIgG-50 Chicken anti Human IgG (H/L) Secondary antibodies
AHIgG-Abs. Chicken anti Human IgG (H/L) Absorbed against Mouse, Goat and Rabbit IgG Secondary antibodies
AHIgG-Abs-B Chicken anti Human IgG (H/L) Absorbed against Mouse, Goat and Rabbit IgG, conjugated with Biotin Secondary antibodies
AHIgG-Abs-F Chicken anti Human IgG (H/L) Absorbed against Mouse, Goat and Rabbit IgG, conjugated with FITC Secondary antibodies
AHIgG-Abs-HRP Chicken anti Human IgG (H/L) Absorbed against Mouse, Goat and Rabbit IgG, conjugated with HRP Secondary antibodies
AHIgG-AGA-1 Chicken anti Human IgG (H/L), conjugated with Agarose Secondary antibodies
AHIgG-AGA-2 Chicken anti Human IgG (H/L), conjugated with Agarose Secondary antibodies
AHIgG-AGA-5 Chicken anti Human IgG (H/L), conjugated with Agarose Secondary antibodies
AHIgG-B Chicken anti Human IgG (H/L), conjugated with Biotin Secondary antibodies
AHIgG-F Chicken anti Human IgG (H/L), conjugated with FITC Secondary antibodies
AHIgG-HRP Chicken anti Human IgG (H/L), conjugated with HRP Secondary antibodies
AHIgGFc Chicken anti Human IgG Fc Secondary antibodies
AHIgGFc-AGA-1 Chicken anti Human IgG Fc, conjugated with Agarose Secondary antibodies
AHIgGFc-AGA-2 Chicken anti Human IgG Fc, conjugated with Agarose Secondary antibodies
AHIgGFc-AGA-5 Chicken anti Human IgG Fc, conjugated with Agarose Secondary antibodies
AHIgGFc-B Chicken anti Human IgG Fc, conjugated with Biotin Secondary antibodies
AHIgGFc-F Chicken anti Human IgG Fc, conjugated with FITC Secondary antibodies
AHIgGFc-HRP Chicken anti Human IgG Fc, conjugated with HRP Secondary antibodies
AHIgM Chicken anti Human IgM (mu chain specfic) Secondary antibodies
AHIgM-B Chicken anti Human IgM (mu chain specfic), conjugated with Biotin Secondary antibodies
AHIgM-F Chicken anti Human IgM (mu chain specfic), conjugated with FITC Secondary antibodies
AHIgM-HRP Chicken anti Human IgM, conjugated with HRP Secondary antibodies
AHLC Chicken anti Human Light Chains (Lambda and Kappa) Secondary antibodies
AHLC-B Chicken anti Human Light Chains (Lambda and Kappa), conjugated with Biotin Secondary antibodies
AHLC-F Chicken anti Human Light Chains (Lambda and Kappa), conjugated with FITC Secondary antibodies
AHLC-HRP Chicken anti Human Light Chains (Lambda and Kappa), conjugated with HRP Secondary antibodies
AMIgGFab Chicken anti Mouse IgG (Fab)2 Secondary antibodies
AMIgGFab-B Chicken anti Mouse IgG (Fab)2, conjugated with Biotin Secondary antibodies
AMIgGFab-F Chicken anti Mouse IgG (Fab)2, conjugated with FITC Secondary antibodies
AMIgGFab-HRP Chicken anti Mouse IgG (Fab)2, conjugated with HRP Secondary antibodies
AMIG Chicken anti Mouse IgG (H+L) Secondary antibodies
AMIG-50 Chicken anti Mouse IgG (H+L) Secondary antibodies
AMIG-AGA-1 Chicken anti Mouse IgG (H+L), conjugated with Agarose Secondary antibodies
AMIG-AGA-2 Chicken anti Mouse IgG (H+L), conjugated with Agarose Secondary antibodies
AMIG-AGA-5 Chicken anti Mouse IgG (H+L), conjugated with Agarose Secondary antibodies
AMIG-B Chicken anti Mouse IgG (H+L), conjugated with Biotin Secondary antibodies
AMIG-F Chicken anti Mouse IgG (H+L), conjugated with FITC Secondary antibodies
AMIG-HRP Chicken anti Mouse IgG (H+L), conjugated with HRP Secondary antibodies
AMIGFc Chicken anti Mouse IgG Fc fragment Secondary antibodies
AMIGFc-AGA1 Chicken anti Mouse IgG Fc fragment, conjugated with Agarose Secondary antibodies
AMIGFc-AGA2 Chicken anti Mouse IgG Fc fragment, conjugated with Agarose Secondary antibodies
AMIGFc-AGA5 Chicken anti Mouse IgG Fc fragment, conjugated with Agarose Secondary antibodies
AMIGFc-B Chicken anti Mouse IgG Fc fragment, conjugated with Biotin Secondary antibodies
AMIGFc-F Chicken anti Mouse IgG Fc fragment, conjugated with FITC Secondary antibodies
AMIGFc-HRP Chicken anti Mouse IgG Fc fragment, conjugated with HRP Secondary antibodies
APIG-B Chicken anti Pig IgG (H+L), conjugated with Biotin Secondary antibodies
ARIG Chicken anti Rabbit IgG (H+L) Secondary antibodies
ARIG-abs Chicken anti Rabbit IgG (H+L), absorbed with human and mouse IgG Secondary antibodies
ARIG-abs-B Chicken anti Rabbit IgG (H+L), absorbed with human and mouse IgG, conjugated with Biotin Secondary antibodies
ARIG-abs-F Chicken anti Rabbit IgG (H+L), absorbed with human and mouse IgG, conjugated with FITC Secondary antibodies
ARIG-abs-HRP Chicken anti Rabbit IgG (H+L), absorbed with human and mouse IgG, conjugated with HRP Secondary antibodies
ARIG-AGA-1 Chicken anti Rabbit IgG (H+L), conjugated with Agarose Secondary antibodies
ARIG-AGA-2 Chicken anti Rabbit IgG (H+L), conjugated with Agarose Secondary antibodies
ARIG-AGA-5 Chicken anti Rabbit IgG (H+L), conjugated with Agarose Secondary antibodies
ARIG-B Chicken anti Rabbit IgG (H+L), conjugated with Biotin Secondary antibodies
ARIG-F Chicken anti Rabbit IgG (H+L), conjugated with FITC Secondary antibodies
ARIG-HRP Chicken anti Rabbit IgG (H+L), conjugated with HRP Secondary antibodies
ASIG Chicken anti Sheep IgG (H+L) Secondary antibodies
ASIG-B Chicken anti Sheep IgG (H+L), conjugated with Biotin Secondary antibodies
ASIG-F Chicken anti Sheep IgG (H+L), conjugated with FITC Secondary antibodies
ASIG-HRP Chicken anti Sheep IgG (H+L), conjugated with HRP Secondary antibodies
ASIG-10 Chicken anti Sheep IgG (H+L), not affinity purified Secondary antibodies
DAIgY Donkey anti Chicken IgY (H+L) Secondary antibodies
DAIgY-B Donkey anti Chicken IgY (H+L), conjugated with Biotin Secondary antibodies
DAIgY-F Donkey anti Chicken IgY (H+L), conjugated with FITC Secondary antibodies
DAIgY-HRP Donkey anti Chicken IgY (H+L), conjugated with HRP Secondary antibodies
DAIgYFab Donkey anti Chicken IgY Fab Secondary antibodies
DAIgYFab-B Donkey anti Chicken IgY Fab, conjugated with Biotin Secondary antibodies
DAIgYFab-F Donkey anti Chicken IgY Fab, conjugated with FITC Secondary antibodies
DAIgYFab-HRP Donkey anti Chicken IgY Fab, conjugated with HRP Secondary antibodies
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