Accelerating the Pace of Discovery

Secondary Antibodies

Secondary antibodies are valuable biological tools that are employed extensively in research applications and diagnostic assays. Secondary antibodies are conjugated to reporter molecules, such as enzymes or fluorochromes, which provide a signal that can be detected and measured. They are commonly used in antibody based applications to detect an antigen specific primary antibody that is not already conjugated to a reporter. Applications that may require a secondary antibody include immunohistology, immunofluorescence, western blotting, enzyme linked immunosorbent assays (ELISA), flow cytometry and lateral flow assays. Careful selection of suitable secondary antibodies also allows researchers to perform simultaneous labelling of multiple targets in a single sample.

A diverse range of monoclonal (Mab), polyclonal (PAb) secondary antibodies are available that vary according to the host, in which the antibody was raised. They are also grouped according to characteristics of the primary antibody recognised with respect to animal species, antibody class and region of the antibody. Secondary antibodies may also be supplied as whole antibodies or as antibody fragments, which can provide technical advantages in some applications.

In addition, antibodies can be conjugated to numerous different reporter molecules thus providing researchers and assay developers with enormous application and assay design flexibility. The correct selection of an appropriate secondary antibody is vital to the success of any immunostaining technique.

Exalpha manufactures and supplies polyclonal secondary antibodies that have been raised in goats and are specific for the Fc portion of mouse IgG. The secondary antibodies recognise all subclasses of mouse IgG and are available conjugated to fluorescein isothiocyanate (FITC) or tetramethylrhodamine isothiocyanate (TRITC). The antibodies are suitable for use in immunocytochemistry, immunofluorescence, immunohistochemistry and ELISA applications.

In addition, Exalpha offers an extensive range of high quality polyclonal secondary antibodies that are suitable for the detection of mouse and rat immunoglobulin classes and subclasses. The range includes secondary antibodies conjugated to horseradish peroxidase, biotin, FITC or TRITC. The antibodies have been tested for Ig cross reactivity and are designed for use in a wide range of research applications.

Code Name Product Type Clone
DAIgY Donkey anti Chicken IgY (H+L) Secondary antibodies
DAIgY-AGA-1 Donkey Chicken IgY, conjugated with Agarose Secondary antibodies
DAIgY-AGA-2 Donkey Chicken IgY, conjugated with Agarose Secondary antibodies
DAIgY-AGA-5 Donkey Chicken IgY, conjugated with Agarose Secondary antibodies
DAIgY-B Donkey anti Chicken IgY (H+L), conjugated with Biotin Secondary antibodies
DAIgYFab Donkey anti Chicken IgY Fab Secondary antibodies
DAIgY-F Donkey anti Chicken IgY (H+L), conjugated with FITC Secondary antibodies
DAIgYFab-B Donkey anti Chicken IgY Fab, conjugated with Biotin Secondary antibodies
DAIgYFab-F Donkey anti Chicken IgY Fab, conjugated with FITC Secondary antibodies
DAIgYFc Donkey anti Chicken IgY Fc fragment Secondary antibodies
DAIgYFc-AGA-1 Donkey anti Chicken IgY Fc, conjugated with Agarose Secondary antibodies
DAIgYFab-HRP Donkey anti Chicken IgY Fab, conjugated with HRP Secondary antibodies
DAIgYFc-AGA-2 Donkey anti Chicken IgY Fc, conjugated with Agarose Secondary antibodies
DAIgYFc-AGA-5 Donkey anti Chicken IgY Fc, conjugated with Agarose Secondary antibodies
DAIgYFc-B Donkey anti Chicken IgY Fc fragment, conjugated with Biotin Secondary antibodies
DAIgYFc-F Donkey anti Chicken IgY Fc fragment, conjugated with FITC Secondary antibodies
DAIgYFc-HRP Donkey anti Chicken IgY Fc fragment, conjugated with HRP Secondary antibodies
DAIgY-HRP Donkey anti Chicken IgY (H+L), conjugated with HRP Secondary antibodies
DoAG/IgG(H+L) Donkey anti Goat IgG (heavy and light chains) Polyclonal
DoAG/IgG(H+L)/7S Donkey anti Goat IgG (heavy and light chains) Polyclonal
DoAG/IgG(H+L)/Bio Donkey anti Goat IgG (heavy and light chains), conjugated with Biotin Polyclonal
DoAG/IgG(H+L)/FITC Donkey anti Goat IgG (heavy and light chains), conjugated with FITC Polyclonal
DoAG/IgG(H+L)/PO Donkey anti Goat IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
DoAG/IgG(H+L)/TRITC Donkey anti Goat IgG (heavy and light chains), conjugated with TRITC Polyclonal
FGAHu/Fab/FITC Goat anti Human IgG Fab, conjugated with FITC Polyclonal
FGAHu/Fab/TRITC Goat anti Human IgG Fab, conjugated with TRITC Polyclonal
FGAHu/IgM(Fc)/FITC Goat anti Human IgM (Fc specific), conjugated with FITC Polyclonal
FGAHu/IgM(Fc)/TRITC Goat anti Human IgM (Fc specific), conjugated with TRITC Polyclonal
FITC-1 Fluorescein Isothiocynate Isomer 1 Secondary Antibodies
FShAHu/IgA(Fc)/TRITC Sheep anti Human IgA (Fc specific), conjugated with TRITC Polyclonal
FShAHu/IgG(Fc)/TRITC Sheep anti Human IgG (Fc specific), conjugated with TRITC Polyclonal
G/PAP Goat Peroxidase-anti-Peroxidase Polyclonal
GA/ConA/PO Goat anti Canavalia Concanavalin A, conjugated with Horseradish peroxidase Polyclonal
GAB/IgG(Fc) Goat anti Bovine IgG (Fc specific) Polyclonal
GAB/IgG(H+L) Goat anti Bovine IgG (heavy and light chains) Polyclonal
GAB/IgG(H+L)/7S Goat anti Bovine IgG (heavy and light chains) Polyclonal
GAB/IgG(H+L)/Bio Goat anti Bovine IgG (heavy and light chains), conjugated with Biotin Polyclonal
GAB/IgG(H+L)/FITC Goat anti Bovine IgG (heavy and light chains), conjugated with FITC Polyclonal
GAB/IgG(H+L)/PO Goat anti Bovine IgG (heavy and light chains), conjugated with Horseradish peroxidase Polyclonal
GAB/TSP/FITC Goat anti Bovine Serum proteins, conjugated with FITC Polyclonal
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