Mouse anti Mouse NKR-P1-A,B,CCatalog number: X1166M
Natural Killer (NK) cells are large, granular lymphocytes found primarily in peripheral blood, where they make up about 10% of the lymphocyte population. Their function is to defend against certain types of tumor cells and virally infected cells. When triggered, the cells release cytotoxic granules which induce either apoptosis or necrosis in the target cell. There are known to be two sets of cell surface receptor on NK cells which recognize target cells with opposing functions. One set, of which an example is NKR-P1 receptors, trigger cytokine release and cell cytotoxicity and the other set, of which Ly49A is an example, inhibit cytokine release. These receptors bind with MHC class I molecules on the surface of healthy cells which inhibits the release of cytokines. In virally infected or cancerous cells, class I MHC molecules are not expressed, inducing the release of cytokine. An activation signal is known to exist, however the mechanics of this signal are not very well understood.
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized (BALB/c X C57BL/6)F1 (CbyB6F1) lymphokine-activated killer (LAK) cells and mouse myeloma cell line.
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Product Form: Unconjugated
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Recommended starting concentration for Western blot is 1-5 µg/ml. Binds to NKR-P1 receptors and induces release of cytokine in SJL/J NK cells.1 Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Kung, S.K.P., et al. 'The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells.' J. Immunol. 1999, 162, 5876-5887 2. Gardiner, C.M. 'Natural killer cells.' Curr. Biol. 1999, 9, R716
Database Name: SwissProt
Accession Number: P27811
Species Accession: Mouse
Safety Datasheet(s) for this product:
Mouse anti Mouse NKR-P1-A,B,C
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