Mouse anti Human T-Complex Protein 1, subunitCatalog number: X2069M
|Clone||F39 P7 F11|
The protein TCP-1 (t-complex polypeptide 1) is a subunit of the hetero-oligomeric complex CCT (chaperonin containing TCP-1) present in the eukaryotic cytosol. The CCT of eukaryotic cytosol is composed of eight different subunit species that are proposed to have independent functions in folding its in vivo substrates, the Actins and Tubulins. TCP-1 was first identified in the mouse as relevant for tail-less and embryonic lethal phenotypes. Sequences homologous to TCP-1 have been isolated in several other species, and the yeast TCP-1 has been shown to encode a molecular chaperone for Actin and Tubulin. TCP-1 found in mammalian cells and yeast plays an important role in the folding of cytosolic proteins.
Synonyms: TCP-1b; Chaperonin containing TCP1, subunit 2 (beta); CCT-beta; CCTB
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminal of the TCP-1 protein and mouse myeloma Ag8563 cells. Sequence common in human, frog, mouse, rat
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formlin-fixed, paraffin-embedded tissue sections (1-5µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Colorectal cancer tissue, Calu6 and HT29 cell lysates
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Kubota, H., et al. (1999). Structures and co-regulated expression of the genes encoding mouse cytosolic chaperonin CCT subunits. Eur. J. Biochem. 262(2):492-500 2. Joly, E.C., et al. (1994). cDNA encoding a novel TCP1-related protein. Biochm. Biophys. Acta. 1217(2):224-226
Database Name: UniProt
Accession Number: P47207
Species Accession: Human
Safety Datasheet(s) for this product:
Mouse anti Human T-Complex Protein 1, subunit
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|"Left and Center: Immunohistochemical staining of normal (left) and cancerous (center) colon tissue using TCP-1β antibody (Cat. No. X2069M). Right: Western blot analysis using TCP-1β antibody on Calu6 (1) and HT-29 (2) cell lysates."|
"Left and Center: Immunohistochemical staining of normal (left) and cancerous (center) colon tissue using TCP-1β antibody (Cat. No. X2069M). Right: Western blot analysis using TCP-1β antibody on Calu6 (1) and HT-29 (2) cell lysates."