Mouse anti Human Matrix Metalloproteinase 2 (MMP2)Catalog number: X2054M
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves Collagen Types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway.
Synonyms: MMP2; MMP-2; CLG4; CLG4A; Matrix metallopeptidase 2; 72 kDa Type IV collagenase precursor; 72 kDa gelatinase, Gelatinase A
Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the C-terminus of the human MMP2 protein and mouse myeloma Ag8563 cells.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Antibody can be used for Western blotting (1-2 µg/ml) and immunohistochemistry on formlin-fixed paraffin-embedded tissue sections (1-5 µg/ml). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Oesophageal and gastric tissues. Expression higher cancerous tissue versus normal tissues.
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Talvensaari-Mattila, A., et al. (2005). Prognostic value of matrix metalloproteinase-2 (MMP-2) expression in endometrial endometrioid adenocarcinoma. Anticancer Res. 25(6B);4101-4105. 2. Honkavuori, M., et al. (2007). MMP-2 expression associates with CA125 and clinical course in endometrial carcinoma. Gynecol. Oncol. 104(1);217-221.
Database Name: UniProt
Accession Number: P08253 (Human)
Species Accession: Human
Safety Datasheet(s) for this product:
Mouse anti Human Matrix Metalloproteinase 2 (MMP2)
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|"A. Immunohistochemical staining of human oesophageal adenocarcinoma tissue using MMP-2 antibody (Cat. No. X2054M). B. Western blot using MMP-2 antibody on recombinant MMP-2 proenzyme (400 ng/lane). "|
"A. Immunohistochemical staining of human oesophageal adenocarcinoma tissue using MMP-2 antibody (Cat. No. X2054M). B. Western blot using MMP-2 antibody on recombinant MMP-2 proenzyme (400 ng/lane). "