Mouse anti Human LEF-1 beta cateninCatalog number: T100M
The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms. Clone REMB1 recognizes the b-catenin binding domain of LEF-1.
Synonyms: lef-1, mouse anti human lymphoid enhancer factor-1, T-cell specific transcription factor 1-alpha, TCF-1alpha
Immunogen: Full length recombinant LEF-1 protein.
Product Form: Affinity Purified
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Antibody can be used for Western blotting (1-5 µg/ml), immunofluorescence on paraformaldehyde-fixed cells (1:1000 or 1 µg/ml), and IHC (paraffin embedded). Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: Jurkat cell lysate
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
Hsu SC; Galceran J; Grosschedl R. 'Modulation of transcriptional regulation by LEF-1 in response to Wnt-1 signaling and association with beta-catenin.' Mol Cell Biol, 18(8):4807-18 1998 Aug
Database Name: UniProt
Accession Number: Q9UJU2 (Human)P27782 (Mouse)
Species Accession: Human
Safety Datasheet(s) for this product:
Mouse anti Human LEF-1 beta catenin
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Figure 1. Immunofluorescence staining of Cos -1 cells transfected with (top to bottom) full length hLEF-1, a LEF-1 mutant missing the nuclear localization signal, TCF-4 and TCF -1. Cells were stained with Exalpha’s LEF-1 antibody, clone REMB1 cat. # T100M and visualized with FITC conjugated mouse IgG. DAPI was used to detect all nuclei. The LEF-1 clone REMB1 recognized an epitope in the beta catenin binding domain that is present only in LEF-1