Mouse anti Human Keratin 13 & 10Catalog number: X1252M
Immunohistochemistry (frozen & paraffin)
Cytokeratins (CK) are intermediate filaments of epithelial cells, both in keratinizing tissue (ie., skin) and non-keratinizing cells (ie., mesothelial cells). Although not a traditional marker for endothelial cells, cytokeratins have also been found in some microvascular endothelial cells. Atleast 20 different cytokeratins (CK) in the molecular range of 40-70 kDa and isoelectric points of 5-8.5 can be identified using two dimensional gel electrophoresis. Biochemically, most members of the CK family fall into one of two classes, type I (acidic polypeptides) and type II (basic polypeptides). At least one member of the acidic family and one member of the basic family is expressed in all epithelial cells. Monoclonal antibodies to cytokeratin proteins can be useful markers for tumor identification and classification.This antibody reacts with 53 kDa and 56.6 kDa cytokeratin (CK) proteins by Western blot. These proteins correspond to CK numbers 13 and 10, respectively, according to Moll's classification1. Using formalin-fixed, paraffin-embedded tissue sections, this antibody detects only the CK13. With frozen sections, this antibody serves as a differentation-related marker for all stratified epithelia and stains all suprabasal cell in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with cytoskeleton preparation from HeLa cells and mouse myeloma cells.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Antibody detects cytokeration protein numbers 13 and 10 (molecular weights 53 and 56.6 kDa, respectively, according to Moll's classification) by Western blot. Does not react with other cytokeratins or other proteins. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Ivanyi, D., et al. Modulation of mammary carcinoma cell phenotype and keratin expression patterns by retinoic acid. Cancer Lett, 1993, 73, 191-205. 2. Ivanyi, D., et al. Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas. Am. J. Vet. Res. 1993, 54, 1095-1102. 3. Ivanyi, D., et al. Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. American Journal of Veterinary Research, 1992, 53, 304-314. 4. Ivanyi, D., et al. Keratin subtypes in carcinomas of the uterine cervix: implications for histogenesis and differential diagnosis. Cancer Research, 1990, 50, 5143-5152. 5. Ivanyi, D., et al. New monoclonal antibodies recognizing epidermal differentiation-associated keratins in formalin-fixed, paraffin-embedded tissue. Keratin 10 expression in carcinoma of the vulva. J of Pathol, 1989, 159, 7-12. 6. Moll, R., et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 1982, 31, 11-24
Database Name: SwissProt
Accession Number: P13646
Species Accession: Human
Safety Datasheet(s) for this product:
Mouse anti Human Keratin 13 & 10
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