Background
The CD36 molecule is an 88kDa transmembrane glycoprotein, which is a member of the class B scavenger receptor family of proteins. Alternative names for CD36 include fatty acid translocase (FAT), platelet glycoprotein 4, platelet glycoprotein IIIb and thrombospondin receptor. CD36 is a pattern recognition receptor that is expressed on a wide variety of cells including monocytes, macrophages, platelets, adipocytes, erythrocyte precursors, activated keratinocytes and some endothelial and epithelial cells. Studies suggest that CD36 has multiple cellular functions and binds to a diverse array of ligands including thrombospondin, modified low-density lipoproteins (LDL), anionic phospholipids and long chain fatty acids. As a scavenger receptor, CD36 plays a key role in mediating the uptake of oxidized LDL by macrophages, the formation of foam cells and the development of atherosclerosis. CD36 is also reported to function as negative regulator of angiogenesis, as a cell adhesion molecule involved in platelet adhesion and aggregation, and as a scavenger receptor in the clearance of apoptotic cells and photoreceptor rod outer segments. Furthermore, CD36 is reported to bind to Plasmodium falciparum infected erythrocytes and may play an important role in the pathogenesis of malaria and sickle cell disease.

Source
185-1G2 is a mouse monoclonal IgG2a antibody derived by fusion of NS1 mouse myeloma cells with spleen cells from BALB/c mice immunized with stimulated human leucocytes.

Product
Each vial contains 100µl 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide. This product is lyophilized

Formulation: Each vial contains 100µl 1mg/ml purified monoclonal antibody in phosphate buffered saline (PBS) containing 0.09% sodium azide. This product is lyophilized

Specificity
The mouse monoclonal antibody 185-1G2 recognises human CD36. The antibody recognises an epitope between amino acids 155 – 183 on the CD36

Applications
The antibody 185-1G2 can be used for CD36 cell identification, quantification and isolation using flow cytometric methods. The antibody is suitable for use in immunocytochemistry and immunohistochemistry on frozen tissue sections. The antibody has also been reported to bind to a site of CD36 that inhibits CD36 function. For this application, please request a sodium azide free antibody format. Optimal antibody dilutions for the different applications should be determined by titration. The recommended range for flow cytometry is 1:100 – 1:200.

Storage
The antibody is shipped at ambient temperature and may be stored at +4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Howard LM, Puente Navazo MD, McGregor JL. Identification of CD36-ractive antibodies that bind an epitope encompassing amino acid residues 155-183. In Leucocyte typing VI. Sixth International workshop and conference. 2. Kishimoto T, Kikutani H, von dem Borne AEG.Kr, Goyert SA, Mason DY, Miyasaka M, Moretta L, Okumura K, Shaw S, Springer TA, Sugamura K, Zola H. Eds. Kobe, Japan, Garland. 1996. p637-638. 2. 3. Englyst NA, Taube JM, Aitman TJ, Baglin TP, Byrne CD. A novel role for CD36 in VLDL-enhanced platelet adhesion. Diabetes 2003; 52:1248 – 1255.

Protein Reference(s)

Database Name: UniProt

Accession Number: P16671

Safety Datasheet(s) for this product:
EA_Sodium Azide