Background
Human Progenitor Cell Antigen (MW 115 K daltons) is found on normal peripheral blood lymphocytes, monocytes, granulocytes and platelets. CD34 is present on 1% or less of cells in normal human bone marrow. Human Progenitor Cell Antigen reacts with 30-70% of acute leukemias, as well as all TdT positive lymphocytes in bone marrow.

Source

Immunogen: CD34=Derived from the hybridization of mouse Sp2/O-Ag14 cells with spleen cells of BALB/c mice immunized with human cell line KG-1a

Product

Product Form: FITC

Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Purification Method: Protein A/G Chromatography

Concentration: Titered for flow cytometry

Applications
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2º to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.

Functional Analysis: Flow Cytometry Staining

Storage
Product should be stored at 4-8ºC. DO NOT FREEZE

Product Stability: See expiration date on vial

Shipping Conditions: Room Temperature

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.

References
1. Antigenic Analysis of Hematopoiesis III. A Hematopoietic Progenitor Cell Antigen Defined by a Monoclonal Antibody raised against KG-1a cells Civin, C.I., Strauss,L.C., Broveal,C., Fackler,M.J., Schwartz,J.F., and Shaper,J.H., J. Immunol. 133,157,1984 2. Rapid isolation of human CD34 hematopoietic stem cells--purging of human tumor cells. Lebkowski JS; Schain LR; Okrongly D; Levinsky R; Harvey MJ; Okarma T Transplantation 1992 May;53(5):1011-9 3. Distribution of surface-membrane molecules on bone marrow and cord blood CD34+ hematopoietic cells. Saeland S; Duvert V; Caux C; Pandrau D; Favre C; Valle A; Durand I; Charbord P; de Vries J; Banchereau J Exp Hematol 1992 Jan;20(1):24-33 4. CD34-positive cell proportions in peripheral blood correlate with colony-forming capacity. Fritsch G; Emminger W; Buchinger P; Printz D; Gadner H Exp Hematol 1991 Dec;19(11):1079-83 5. CD34 expression by stromal precursors in normal human adult bone marrow. Simmons PJ; Torok-Storb B Blood 1991 Dec 1;78(11):2848-53 6. Human CD34+ HLA-DR- bone marrow cells contain progenitor cells capable of self-renewal, multilineage differentiation, and long-term in vitro hematopoiesis. Srour EF; Brandt JE; Briddell RA; Leemhuis T; van Besien K; Hoffman R Blood Cells 1991;17(2):287-95 7. Surface marker expression in acute myeloid leukaemia at first relapse. Thomas X; Campos L; Archimbaud E; Shi ZH; Treille-Ritouet D; Anglaret B; Fiere D Br J Haematol 1992 May;81(1):40-4 Product Specific References: 1. Gardner, J.P. et al. 'Robust, but transient expression of adeno-associated virus-transduced genes during human t lymphopoisis.' Blood, 1997, 90, 4854-4864.

Protein Reference(s)

Database Name: UniProt

Accession Number: P28906

Safety Datasheet(s) for this product: