Mouse anti Human Caspase-8Catalog number: X1176M
Caspases are key effectors of programmed cell death. They are synthesized as inactive proenzymes which are activated by cleavage at a specific aspartate residue to form two subunits. These subunits are normally linked together by a linker which may be involved in the regulation of the different caspases. Caspase-8 is a key enzyme in the apoptosis pathway. Caspase-8 contains two N-terminal region death effector domains which are removed to activate the enzyme along with cleavage into the two subunits. These subunits then for the active protease which is capable of cleaving caspase-3, -6 and -7. These caspases are then capable of cleaving other cellular substrates such as PARP and DFF, which in turn induce apoptosis.
Synonyms: Cysteine-requiring Aspartate Protease-8; Apoptotic protease Mch-5; ICE-like apoptotic protease 5; FADD-like ICE
Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human capase-8 protein and mouse myeloma cells.
Product Form: Unconjugated
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
Purification Method: Protein A/G Chromatography
Concentration: See vial for concentration
Detects proenzyme form of human Caspase-8 by Western blot analysis as well as one of the cleavage products. Optimal concentration should be evaluated by serial dilutions.
Functional Analysis: Western Blotting
Positive Control: MCF-7 cell lysate
Product should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
Product Stability: See expiration date on vial
Shipping Conditions: Ship at ambient temperature, freeze upon arrival
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but Exalpha Biologicals accepts no liability for any inaccuracies or omissions in this information.
1. Cohen, G.M., et al. Caspases: the executioners of apoptosis. Biochem. J. 1997, 326, 1-16 2. Martin, D.A., et al. Membrane oligomerization and cleavage activates the caspase-8 (FLICE/MACHalpha1) death signal. J. Biol. Chem. 1998, 273, 4345-4349 3. Green, D.R., Apoptotic pathways: the roads to ruin. Cell 1998, 94, 695-698 4. Hu, S., et al. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J. Biol. Chem. 1997, 272, 17255-17257
Database Name: SwissProt
Accession Number: Q14790
Species Accession: Human
Safety Datasheet(s) for this product:
Mouse anti Human Caspase-8
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|Western blot analysis using caspase-8 antibody on MCF-7 cells negative (-) and positive (+) for caspase-3 after treatment for 48 hours with thapsigargin. Antibody detects the proenzyme and one of the cleavage products.|
Western blot analysis using caspase-8 antibody on MCF-7 cells negative (-) and positive (+) for caspase-3 after treatment for 48 hours with thapsigargin. Antibody detects the proenzyme and one of the cleavage products.