Evaluation of cell cycle progression is essential for investigations in many scientific fields. Measurement of [3H] thymidine incorporation as cells enter S phase has long been the traditional method for the detection of cell proliferation. Subsequent quantification of [3H] thymidine is performed by scintillation counting or autoradiography. This technology is slow, labour intensive and has several limitations including the handling and disposal of radioisotopes and the necessity of expensive equipment.

A well-established alternative to [3H] thymidine uptake has been demonstrated by numerous investigators. In these methods bromodeoxyuridine (BrdU), a thymidine analogue replaces [3H] thymidine. BrdU is incorporated into newly synthesized DNA strands of actively proliferating cells. Following partial denaturation of double stranded DNA, BrdU is detected immunochemically allowing the assessment of the population of cells, which are actively synthesizing DNA.

Apoptosis, also known as programmed cell death or ankoikis, leads to the elimination of cells without releasing harmful substances into the surrounding area. Apoptosis is the result of a cascade of molecular and biochemical events involving endogenous endonucleases that cleave DNA into the prototypical ‘ladder of DNA fragments’. In addition to producing classical DNA ladders, the apoptotic endonucleases generate free 3’-OH groups at the ends of these DNA fragments to allow for the detection of apoptotic cells using a molecular biology-based, end-labelling technique.

Kit Manuals

Our extensive range of kits can support your research whatever your area of interest.

Code Name Product Type Clone
X1327K1 BrdU Cell Proliferation Assay Kit Assay Kit
X1327K2 BrdU Cell Proliferation Assay Kit Assay Kit
X1327K3 BrdU Cell Proliferation Assay Kit Assay Kit
X1623K1 BrdU Chemiluminescent Cell Proliferation Assay Kit Assay Kit
X1623K2 BrdU Chemiluminescent Cell Proliferation Assay Kit Assay Kit
X1623K3 BrdU Chemiluminescent Cell Proliferation Assay Kit Assay Kit
X1545K BrdU Immunohistochemistry Kit Immunohistochemistry Kit
X1053 ClonoPath(tm) – CD38/CD56/CD19 Flow Cytometry Kits
X2044K1 DNA Fragmentation Detection Kit Immunohistochemistry Kit
X2044K2 DNA Fragmentation Detection Kit Immunohistochemistry Kit
IK 2000 EggsPress IgY Purification Kit (20 yolks) Kits
IK 4000 EggsPress IgY Purification Kit (40 yolks) Kits
IK 500 EggsPress IgY Purification Kit (5 yolks) Kits
IK 8000 EggsPress IgY Purification Kit (80 yolks) Kits
KL6-ES243905 KL-6 ELISA (Sialylated Carbohydrate, MUC1)
AE002 Peroxidase Substrate Kit AEC, 2 Components
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