Catalogue

Background
The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. It does not react with any non-Ig protein in turkey serum, as tested by immunoelectrophoresis and double radial immunodiffusion.

Source
Purified normal IgG isolated from pooled turkey serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Product
Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline. No preservative added. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

Product Form: Biotin

Formulation: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline. No preservative added.

Specificity
Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Turkey IgG, heavy and light chains.

Species Reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, however in immunoelectrophoresis a precipitin line with pigeon, Duck and Chicken has been observed.

Applications
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Storage
The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Caution
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. It may contain hazardous ingredients. Please refer to the Safety Data Sheets (SDS) for additional information and proper handling procedures. Dispose product remainders according to local regulations.This datasheet is as accurate as reasonably achievable, but our company accepts no liability for any inaccuracies or omissions in this information.