Exalpha Biologicals, Inc.

Product Highlight

FIX&PERM Cell Fixation and Permeabilization Kit

Flow cytometric analyses with monoclonal antibodies were so far mainly restricted to cell surface molecules. Intracellular structures such as cytoplasmic or nuclear enzymes, oncoproteins, cytokines, immunoglobulins etc. were largely excluded from such studies. Also excluded from flow cytometric studies were cytoplasmic localizations of well-established membrane molecules like CD3 and CD22, which, in their cytoplasmic form, are the most reliable lineage markers in undifferentiated leukemia. With the FIX&PERM® Kit flow cytometric analysis of intracellular antigens has become as easy as surface antigen studies. The only prerequisite is the availability of suitable antibody conjugates. Most of the available monoclonal antibody conjugates can be used with the FIX&PERM® Kit, some determinants are sensitive, however, to the fixation step involved. This and the optimal fixation time have to be tested for each reagent.

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Welcome, Peter Rutten

We are pleased to announce that Peter Rutten has now started in his role as Operations Director here at Exalpha Biologicals Inc. Peter has a Master’s of Science degree in Industrial and Organizational Psychology and this has lead him down a very business orientated career path. Peter is looking forward to working with the team at Exalpha. Peters primary focus is the customer experience and he will be working with the Laboratory team, the Quality Control team and the Order Processing team to ensure this focus is achieved. We all wish Peter well in his new role.

Exalpha Biologicals, Inc.

c-myc

  • Product Code: n115m
  • Size: 100 µg
  • Availability: In Stock In Stock
  • Price (USD): $244

Cat #

n115m		 Quantity:      

Product Name

c- myc

Synonyms

cmyc; Myc proto-oncogene protein; transcription factor p64

Host/Source

Mouse

Clone

33

Isotype

IgG1

Product Type

Monoclonal Antibody

Reactivity

Human, Mouse, Avian

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry

Purification

Protein A/G Chromatography

Size

100 µg

Price (USD)

$244

Background

Human c-myc belongs to a family of 5 myc oncogenes. Amplification of c-myc has been found in several types of human tumors including breast and colon carcinomas. Induction of c-myc in cells grown in the appropriate growth factors or co-expression with survival genes, such as bcl-2 causes cell proliferation. In the abscence of these factors, c-myc expression causes cell death. c-myc gene gives rise to at least two nuclear phosphoproteins of 64kDa and 67kDa, the unphosphorylated form is 49kDa.

Immunogen

Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant full-length human c-Myc protein and mouse myeloma cells.

Formulation

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Customer Storage

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

Target Molecular Weight

48.8

Database Links:

SwissProtP09416Rat
SwissProtP01108Mouse
SwissProtP01106Human

References

1) K. Alitala et. al. (1983) Proc. Natl. Acad. Sci., 80: 1707-1711

2)H. Person et.al. (1984) Science 225 687-693